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Russian Journal of Parasitology

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No 3 (2014)
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FAUNA, MORPHOLOGY AND SYSTEMATICS OF PARASITES

7-11 334
Abstract

Helminthological studies of carnivores in Central Black Earth Nature Reserve (Kursk region) are carried out. High degree of infection of carnivores with hel-minthes is determined. Helminth eggs have been detected in 98 % of animal fecal samples. More often eggs of cestodes, nematodes Capillariidae, Tosascaris leoni-na, Uncinaria stenocephala, trematodes Alaria alata have been found. In 94,1 % of foxes a mixed infection caused by A. alata, Capillaria spp., T. leonina, Toxocara sp., U. stenocephala and cestodes has been determined. Carnivores of martens fam-ily were infected with Capillaria spp. and cestodes. 4,9 % of foxes were infected with one helminth species. The most common helminthes in carnivores are Capil-laria spp. High density of carnivores in nature reserve may cause widespread hel-minthosis.

 

ECOLOGY AND BIOLOGY OF PARASITES

12-14 256
Abstract
Production of Trichocephalus ovis eggs in sheep body is studied. Estimation of egg production is carried out by division of the amount of Trichocephalus eggs released from animals feces within one day into the amount of Trichocephalus detected after sheep slaughtering. The maximum reproductive capacity of females T. ovis develops in spring and summer. In winter (January) T. ovis egg production significantly slows down in comparison with the spring period. Fertility of T. оvis in sheep body was in winter 3140,6±214,8 expl./day, in spring 3978,4±245,7, in summer 4291,4±317,3 and in autumn 5572,3±384,2 expl./day. High T. ovis egg production may cause a wide spread of trichocephalosis in sheep in Chechen Republic.

EPIZOOTOLOGY, EPIDEMIOLOGY AND MONITORING OF PARASITIC DISEASES

15-19 260
Abstract
Distribution and extent of manifestation of diseases of an integument in dif-ferent climatic zones of North Caucasus region in 2001–2010 is studied. Dermatitis of parasitic etiology noted at 88–91 % of sheep in different zones of the region. The maximum contamination of sheep by ectoparasites was in droughty zone. Dermatitis of noncontagious and infectious etiology is established respectively at 5–6 and 4–5 % of sheep. In 2001–2010 the frequency of disease of skin of parasitic etiology significantly didn't change. Most sheep were struck with Psoroptes ovis and insects of Melophagus ovinus. 47 % of sheep are infected with ectoparasites. Damages of skin noted at sheep during all seasons. In the first half of winter the number of sick sheep raised, especially at the dense contents. Among young growth skin disease extend quicker. Preventive treatment of sheep cover 20–50 % of livestock and they are carried out within the year. 45 % of processings of sheep carry out in winter.
20-23 300
Abstract
Trichocephalosis is widespread in sheep everywhere on the territory of the Russian Federation. Distribution of trichocephalosis in sheep in different zones of the Chechen Republic and age dynamics of their infection by Trichocephalus spp. is studied. Contamination of sheep by Trichocephalus spp. studied on the basis of research of feces and post mortem investigations of intestines. In the conditions of east part of Central Caucasus Mountains the wide circulation of trichocephalosis in sheep is established. Contamination of sheep made, on the average, by Trichoceph-alus ovis of 16,71 %, T. skrjabini of 6,2 % at intensity of infection respectively 42,6±5,6 and 12,3±3,4 expl./goal. Extensiveness of the infection made in a flat zone 11,5 %, foothill – 19,8 and a mountain zone – 16,7 %. Contamination of sheep with age considerably decreases. Sheep had an equal extensiveness of the infection aged about year of 23,0 %, 1–2 years 19,4, 2–4 years – 13,6 and is more senior than 4 years – 9,5 %. Maximum contamination is established in sheep at the age of 6–12 months.
24-29 228
Abstract
Epizootic situation on piroplasmosis in cattle is clarified and the species com-position of ixodid ticks parasitizing animals in conditions of the republic of Dage-stan is determined. The species composition of piroplasma, its proportion as well as dynamics of diseases of cattle are identified by examination of blood films taken from 289 head of cattle with piroplasmosis. The efficacy of chemotherapeutic drugs is studied on animals spontaneously infected with Piroplasma bigeminum, Francaiella colchika and Theileria annulata. In the territory of the examined farms piroplasms P. bigeminum, F. colсhika, T. annulata, A. marginaliа and ixodid ticks Boophilus calcaratus, Rhipicephalus bursa, Hyalomma anatolicum, H. scupenze are most common. Neozidin is applied for treatment of cattle with fransaiellesis, piroplasmosis and babesiosis. For treatment of cattle with theileriosis the following drug combination should be applied: Sulfantrol + Neozidin or Sulfantrol + Veriben with simultaneous use of antibiotics.
30-33 295
Abstract
Spread of common helminthosis in herd horses in conditions of Republic Sakha (Yakutia) on the base of coproovoscopy using Fülleborn's method and К.I. Skryabin method of full helminthological autopsy is studied. Young horses were infected with strongyle eggs on the first few days on pasture and started releasing helminth eggs with feces on the end of July. Infection peak has been registered in September. Delafondia sp., Alfortia edentatus and Strongylus vulgaris eggs have been detected at first in feces of young horses in October, December and February respectively; peak of infection has been determined in December–February, Febru-ary and April. 63,8 % of young horses were infected with Parascaris equorum at intensity of infection 13,2±1,1 expl. In some farms 100 % of horses were infected with P. equorum. The maximum P. equorum egg production has been registered in summer and autumn and was respectively 11,7 thousand and 11,2 thousand expl/day. Peak of infection was in October–December, and in winter the infection rate decreased. 70 % of horses and 80 % of young horses were infected with Ano-plocephala perfoliata in October. Application of Equisect showed a 100 % effica-cy in treatment of strongyloidosis and oxyurosis in horses. Strongyle eggs overwin-tered and remained viable in spring. P. equorum eggs don’t remain viable in win-ter.
34-37 198
Abstract
Prevalence of ticks of the genius Scheloribates, Berlese, 1908 and Galumna, by von Heyden, 1826 and their infection with Moniesia spp. cysticercoids on vari-ous types of pastures of the Vologda region is studied. Density of population of ticks of the genius Scheloribates and Galumna made in the soil of shelters for ani-mals 714±47,0, natural forest pastures 312±31,0, natural meadow pastures 167±14,0, artificial cultural pastures 114±8,0, haymaking places under hay rolls 122±18,0 expl./m2 at detection of Moniesia spp. cysticercoids respectively at 50,1; 26,9; 22,8; 0 and 13,9 % ticks. The maximum number of ticks is revealed in July–August in shelters of summer camps for animal and natural forest and meadow pas-tures, and the smallest – on artificial cultural pastures. Animals in shelters for over-exposure are subject to the maximum risk of infection with moniesiosis.
38-47 214
Abstract
The most important parameters of infection transmission: vector competence and vector ability of transmitters, elements of genesis of vector-borne diseases and intrabody pathology of insects are briefly described. Emergent occurrence and spread of vector-borne exotic diseases in nonendemic areas can be considered as a precursory warning of pandemic threat caused by infectious agents including pre-viously unknown agents of new areas and ecological zones. Epidemiological sig-nificance of arboviral mosquito-borne infection is being discussed.
48-51 295
Abstract
Distribution of onchocercosis in the conditions of East Caucasus is studied by the method of dermolarvoscopy of 199 horses and helminthological autopsy of a cervical sheaf and sinews of extremities of 118 horses. Onchocercosis is wide dis-tributed in Chechen Republic. The extensity of infection of horses with Onchocerca spp. on areas fluctuates from 23,0 to 41,6 % that, on average, makes 32,66 % at detection in 100 mg of skin of 95,2–181,4 expl. of microfilariae. Extensiveness of the infection caused by Onchocerca cervicalis, O. reticulatа and both types made respectively 25,42 %, 5,93 and 2,54 %. The maximum contamination of horses is noted in foothill zone. Contamination of horses with age considerably raises. Max-imum contamination is established in horses at the age of 6–8 years. It is noted dif-ferences in age dynamics of horses infection with O. cervicalis and O. reticulatа.

PATHOGENEZIS, PATHOLOGY AND ECONOMIC DAMAGE

52-58 187
Abstract
On applying the method of alkaline Single Cell Gel Electrophoresis (SCGE) it has been established that metabolites of cat liver fluke maritas have a genotoxic influence on somatic cells of golden hamsters. Genotoxic influence is observed in blood cells of animals on the 7, 14, 21, 28, 35, 60, 90, 120 and 150-th days of inva-sion with the maximum 8,2 times expression on the 14-th day of invasion. The in-dicator «the comets tail moment» in bone marrow cells was 1,9–6,5 times higher than the control values with the maximum expression on the 21-st day of invasion. Maximum genotoxic 7,1 times effect in the liver was observed on the 14-th day of invasion. The level of apoptotic cells in blood, bone marrow and liver of animals in experimental opistorchosis increases due to cytotoxic effect of invasion. Cytotoxic effect of metabolites of cat liver fluke maritas is observed in blood on the 7, 14, 21, 28, 35, 60, 90, 120 and 150-th days with the maximum 8,3 times expression of these changes on the 21-st day. In bone marrow the maximum 10,5 times degree of cells apoptosis was observed on the 14 and 28-th days of invasion. The liver cells apoptosis in infected animals 2,3-6,2 times exceeded the control values with the maximum expression of these changes on 28-th day of observation.
59-62 227
Abstract
Based on above mentioned the authors set a goal to determine the bioavailabil-ity of meat in relation to its chemical composition depending on mono- and mixed infections in cattle. Studies are conducted on 20 animals that according to results of coprological research and helminthological autopsy by K.I. Skryabin method are divided into 4 test groups (5 heads in group). Animals in first group were infected with Fasciola sp., second group – Dicrocoelium sp., third group – association of Fasciola sp. and Dicrocoelium sp., fourth group – control group (intact group). Based on research results the authors came to conclusion that in case of trematodo-sis the changes of chemical composition and chemical physical properties of meat especially of beef registered by trematodosis may result in degeneration of quality and nutritional value of the most valuable food stuff. It is especially pointed out that the dry-substance content is reducing due to presence of mineral components. Moreover the protein fat ratio in helminthosis is less than the value of control group. This is especially expressed in dicrocoeliosis mono infection that may be caused by intoxication of animal and disturbance of proteins and fats metabolism. Infection with trematodes affect the chemical composition and reflect the nutrition-al value of meat that has been proven by reducing (Р < 0,001) of food energy by 52,49–66,47 kJ as well as by accumulating of products of decomposition of organic compounds verified by рН value and coefficient of acidity – oxidation of meat.
63-67 219
Abstract
Micromorphological changes in placenta tissues of tracker dogs spontaneously infected with Toxascaris leonina are studied. Microscopic study enables to esti-mate the following changes occurring in placenta: involutive changes, adaptive compensatory changes, blood flow disturbances and inflammations. As a result of conducted studies it was determined that T. leonina causes alternative processes in dogs' placenta: dystrophy of decidual cells, of choroid epithelium up to formation of necrosis; blood flow disorders at the utero-placental level; change of thickness of barrier between maternal and fetal blood circulation; immunopathological changes, not complete adaptive compensatory processes. The above mentioned changes result in hypotrophy and hypoxia of fetus.

BIOCHEMISTRY, BIOTECHNOLOGY AND DIAGNOSTICS

68-37 414
Abstract
The comparative assessment of efficiency of the coprologic methods of diag-nosis of helminthosis is given: MakMaster, Kotelnikov–Hrenov and Mini Parasep concentrator at an artificial laying of eggs in feces. The description of these meth-ods of diagnostics is in detail provided. It isn't established differences in efficiency of methods of MakMaster and Kotelnikov–Hrenov with use of the calculating chamber which appeared more effective, than the Mini Parasep concentrator. At a laying of 20 expl. of eggs of helminths in 1 g of feces of egg aren't found in 60 % of tests by MakMaster's method, in 63 % – by method Kotelnikov–Hrenov and in 72 % – by Mini Parasep method. At concentration of 50 eggs in 1 g of feces Makmaster and Kotelnikov-Hrenov's method didn't find eggs in 36 % of tests, and by Mini Parasep method – in 42 % of tests. At concentration of 200 and 300 eggs in 1 g of feces eggs are found in tests by all three methods. Methods of researches of feces are more exact at concentration of 100 and more eggs in 1 g of feces. With increase of concentration of the eggs brought in feces efficiency of methods in-creased. The Mini Parasep method which showed efficiency in the presence of more than 200 eggs in 1 g of feces appeared less exact. Sensitivity of a method var-ied from 0 to 81 % depending on concentration of eggs in tests of feces that points to its insufficient efficiency. Existence of foreign particles in the studied tests of feces complicates research of tests and therefore some eggs of helminths can be not found.
74-76 207
Abstract
The method of allocation of Toxocara canis larvae from parenchyma of liver and lung of carnivorous animals for postmortem diagnostics of toxocarosis is of-fered at a weak infection and in prepatent period. The received culture of larvae of T. canis can be used for studying pathogenesis of the diseases, carrying out genetic researches and receiving proteins with diagnostic and protective properties. The list of the equipment, reactants and solutions for allocation of T. canis larvae is given. The work course which consists of preparation of tests of tissue of a liver and lungs, preparation of artificial gastric juice, digestion of tissues, an assessment of the re-sults, concentration of T. canis larvae is described. For researches take samples of a parenchyma of lungs or a liver weighing 50 g, crush on a meat grinder. Samples digest to the current of 50 min. at a temperature of 41–42 °C. After 10 minute up-holding a deposit merge in Petri's cups and investigate on existence of T. canis lar-vae and their mobility. For concentration of a material a deposit centrifuge during 10 min.

TREATMENT AND PREVENTION

77-80 375
Abstract
Data on efficacy of application of Veriben in specific and symptomatic treat-ment of cattle naturally infected with Babesia bovis are presented. It was deter-mined that a twice intramuscular injection of Veriben to cattle in the dose of 1 ml per 20 kg of body weight with an interval of 24 hours provides an effective treat-ment. The lower doses did not show a good efficacy. Symptomatic treatment pro-vides more quickly recovery of sick animals. In the animals of the 1st group after the 1st injection of Veriben was observed a sharp decrease of parasitemia and after the 2nd injection no parasites in blood were detected. Drop of body temperature to normal and improvement of welfare of animals were also observed. While in the animals of the 2nd and 3rd experimental groups despite the reduced parasitemia, babesia remained in the blood, the body temperature was still increased and the clinical signs of babesiosis did not disappear.
81-85 236
Abstract
Development of livestock as well as increase of milk and meat production depends considerably on prompt and qualified veterinary measures. One of the reserves of lifestock profitability rise is prevention of infectious diseases including entomosis, and animals' protection from bloodsucking dipterous insects: deer flies, mosquitos, gadflies and biting midges also known as gnats. In Belorussia dairy cows lost up to 15–20 % of milk and in single seasons up to 30–40 % due to bloodsucking dipterous insects attack. The task of present research is the study of insecticide efficacy of drugs: Delcid, Almet, Fenmet, Breeze 25 % applied as aerosols using ultra low-volume spraying when cattle in pastures are massively attacked by gnats. The repellent efficacy of drugs Breeze-Prof, Polevik, Phytolym, Tobol and Alezan spray against gad-flies are studied. While using of ultra low-volume hitch spraying of young cattle herds: 0,05 % solution of Delcid, Almet, Fenmet, Breeze 25 % at the rate of 10 ml per cattle it is found that the protective action coefficient according to average data related to gadflies family Tabanidae within first 30 minutes makes: for Delcid – 92,49 %, Almet – 100, Fenmet – 100, Breeze 25 % – 95,3 %, and within 24 hours: Delcid – 81,7 %, Almet – 71,4, Fenmet – 56,2, Breeze 25 % – 81,8 %.
86-90 248
Abstract
The purpose of our study was the evaluation of time of knockdown effect on insects, and of the height the ticks climb to on the clothing treated with prepara-tions «RolfClub 3D drops for dogs» and «RolfClub 3D drops for cats». «RolfClub 3D drops for dogs» and «RolfClub 3D drops for cats» contain respectively the fol-lowing acting agents: Fipronil, D-сifenotrin, Pyriproxyfen and Fipronil, Etofenprox, Pyriproxyfen. Medications are solutions applied in practice on skin singly «spot on». It was determined that the tested medications have a highly ex-pressed knockdown effect, the time of knockdown effect by applying of «RolfClub 3D drops for dogs» is 2,16±0,23 min, «RolfClub 3D drops for cats» – 2,03±0,23 min by average climbing height respectively 21,4±2,16 and 25,2±2,3 cm.

PHARMACOLOGY, TOXICOLOGY

91-95 234
Abstract
Subacute toxicity of Ricazol as injection, containing in 1 ml 100 mg Rico-bendazole is studied. The preparation in doses of 125 and 50 mg/kg at daily intro-duction during 7 days didn't cause deviations in behavior, consumption of forage and water, hematologic and biochemical indicators, mass coefficients of bodies, a functional condition of rats. Introduction Ricazol in a dose of 250 mg/kg reduced additional weights and body weight, caused oppression of animals and a case, changes of the main hematologic and biochemical indicators, including erythro-cytes, leukocytes, hemoglobin, bilirubin, urea and activity alanine aminotransfer-ase. The dose of ricazol of 250 mg/kg is toxic.
96-101 263
Abstract
Acute toxicity of Ricazol based on Ricobendazole by oral administration and intramuscular injection in white outbread mice and rats as well as by dermal ad-ministration in white rats is studied. To test the parameters of acute oral toxicity of Ricazol the compound was administered singly to experimental animals (at various doses). General well-being and behavior of animals, manifestation of intoxication symptoms and possible death of animals were observed within 14 days. Post-mortem examination of dead animals was carried out. To investigate acute skin toxicity of Ricazol the compound was given singly to experimental animals on the skin area (size 5cm × 5cm) at the doses of 10000, 15000 и 20000 mg/kg of active ingredient. LD50 value by injection into the stomach for mice was 10000 mg/kg, for male rats – 2500, female rats – 2700 mg/kg. LD50 by dermal administration in rats was 11200 mg/kg, by intramuscular injection in rats – 2500, in male mice – 4900 mg/kg.
102-106 248
Abstract
Subacute toxicity of the injective anthelmintic drug Ritril containing in 1 ml 50 mg ricobendazole and 50 mg triclabendazole has been studied on rats. The med-ication applied by intramuscular injection at the doses of 40 and 100 mg/kg did not have any effect within 7 days on clinical status, consumption of food and water, hematological and biochemical values, mass coefficients of the organs and func-tional status of the examined animals. Verifiable changes of estimated values have been registered within 7 days by intramuscular injection of Ritril at a dose of 200 mg/kg. Change in relative liver and lungs weight, decrease of amount of erythro-cytes and hemoglobin in rat’s blood, verifiable change of liver values: total and direct bilirubin levels, amounts of aspartate aminotransferase and alanine ami-notransferase, and increase of blood urea concentration in rats are determined.
107-111 211
Abstract
Immunotoxic properties of 5 % suspension of kokcidon in two tests are stud-ied. By results of reaction of hypersensitivity of the slowed-down type the reaction index at the animals receiving a preparation in a therapeutic dose of 0,4 ml/kg, has made 11,46, at the animals receiving a 10 times increased dose – 11,31, and at an-imals of control group – 14,26 that allows to draw a conclusion on lack of oppress-ing effect of 5 % suspension kokcidon on the T-cellular link of immune system. A single skin application of tested drug in a therapeutic and tenfold increased thera-peutic doses doesn't cause changes of intensity of antibody production against a thymus-dependent antigen. The index of action of a preparation in both doses made 0,98. Reaction of gemagglyutination didn't reveal negative influence of a prepara-tion on the humoral immune answer.
112-117 520
Abstract
The toxicological assessment is given and efficiency of complex preparations is studied. «RolfClub 3D spray for dogs» contains fipronil, D-cifenotrin, piriproxi-fen; «RolfСlub 3D spray for cats» – fipronil, etofenprox, piriproxifen. Preparations represent solution and are applied spot on as spray. Parameters of acute and skin toxicity of preparations for dogs and for cats are determined. LD50 at introduction in a stomach to mice made 5809±508,6 and 4295±607 mg/kg respectively, LD50 when drawing on skin to rats – more than 30000 mg/kg (the 4th class of danger). Preparations don't possess local irritating action. At application in twice and five time increased doses during 7 days have no negative impact on the general condi-tion of dogs and cats, their physiological status and behavior, don't change morpho-logical structure and biochemical indicators of blood and urine. Preparations pos-sess accurately expressed insektoakaricid activity on all stages of development of fleas, louses, ticks of the genius Sarcoptes and Demodex. Preparations don't cause side effects and complications.

PARASITES OF PLANTS

118-121 249
Abstract
Parasitic fauna of grapes in Martuninsk District of Nagorno-Karabakh is in-vestigated. Soil samples were collected at the deepness of 10–20 and 20–40 cm. Release of nematodes from soil was carried out using standard methods. Plant roots were examined for nematodes under a binocular microscope. Identification of the genus and species was conducted using temporary and permanent glycerin drugs. Parasitic fauna of examined grapes is represented by 5 genera (Xiphinema, Lon-gidorus, Helicotylenchus, Rotylenchus, Macroposthonia). 7 species were indenti-fied: Helicotylenchus digonicus, H. dihistera, H. pseudorobustus, Macroposthonia xenoplax, Xiphinema brevicolle, X. index, X. pachtaicum. Nematodes Xiphinema – transmitters of viruses are detected in rhizosphere of damaged grape plants; these plant-parasitic nematodes are of a great economic importance because of their spe-cific pathogenic effect; besides their direct harmful effect on plant’s root system they transmit various virus infections: grapevine leafroll virus, Arabis mosaic virus etc.
122-129 258
Abstract
Methods of monitoring of cyst nematodes using telecommunication technolo-gies, aerospace photography, as well as the traditional route investigation of agro-cenosis have been improved. Terms, sequence of monitoring implementation steps and perspectives of practical use of different cartograms of nematodosis have been proved. It is necessary to select and analyze plant samples on all four field sides. 5 plants from each sample are investigated on a rectangular field. In relation to culti-vated crops, perennial legumes – 100 plants from each field were studied. A me-chanic sampler device is being used for plant sampling that allows to reduce 10 times labor costs. Aerial photography of the earth surface including fields enables to detect the centres of plant infection. Aerospace monitoring of plant nematodosis is being conducted at the end of June – in the first and second decades of July. For more precise examination small aircraft and remote sensing unmanned planes with digital cameras are being used. Spectral analysis of pictures allows to detect nidi of plant damage by nematodes. To determine degree of plant damage and species composition of nematodes a route examination of fields are conducted, cartograms created, forecasts fulfilled, electronic cards of cyst nematode spread created.
130-136 249
Abstract
Species composition, injuriousness and spread of most dangerous phytopara-sites in European part of RF are determined. Research studies have been conducted in Stavropol and Krasnodar Krai, Rostov region, Kabardino-Balkaria Republic, Dagestan and Chechnya, in Central Region – Moscow, Ryasan and Tula regions, Central Black Earth Region – Volgograd region, Komi and Karelia republic. As a result of phytoparasitological examination of fruit and berry trees in these areas the complex (mixed), viral, bacterial, fungal (including oomycetes) and nematode in-fections as well as ticks have been detected. It was determined that the increase of amount of hazardous organisms and their species composition occurs from North to South and has a pronounced epiphytotic nature especially in case of complex infection of plants with viruses, bacteria, fungi, oomycetes and nematodes. It was pointed out that fungal, bacterial and viral infections associated with endo- and ec-toparasitic nematodes may cause mass withering and shrinking of tree shoots and dying of fruit plants, development of flat-headed skeletal branches and mass mor-tality of large fruit trees.

METHODOLOGICAL REGULATIONS

137-140 220
Abstract
It is established that on the Arctic coasts the causative agent of trichinellosis is Trichinella nativa. Trichinella circulate in populations of wild predatory animal land and water ecosystems, among the sinantropic and pets, and also animals of cellular cultivation, when conducting traditional economic activity of indigenous people which promotes maintenance natural the center. The main ways of transfer Trichinella in populations of animals are necrofagiya, predatoriness and cannibal-ism with the participation of numerous animals-disseminators. The Arctic isolates of Trichinella are characterized by specific signs thanks to which they adapted for low temperatures and other abiotic factors. Indigenous people regularly uses in food thermally raw meat of wild animals, thereby constantly subjecting itself to risk of infection with a parasite. The most important applied aspect of trichinellosis is its prevention and infection monitoring taking into account traditional environ-mental management of the native population.
141-144 196
Abstract
Methods for interspecific and intraspecific differentiation of helminthes, iden-tification of genotype Trichinella spiralis using polymerase chain reaction are de-veloped. These methods detect DNA loci specific for genome T. spiralis and ena-bles to determine connection between natural and synanthropic trichinellosis in different biocenosis. Methods are based on interaction of synthesized oligonucleo-tide primers with DNA loci typical for this parasite genotype. The amplified frag-ment length is increasing. Electrophoresis of amplification products enables to vis-ualize the DNA fragments and find their molecular mass. The list of equipment and reagents is presented, the process of preparation of clinical trial materials, release of genomic DNA, preparation of reaction mixture for polymerase chain reaction, amplification and electrophoresis modes are described
145-150 180
Abstract
The analyze of epizootic and epidemiological situation indicates an increased role of wild animals in spreading of such zoonotic diseases as trichinellosis and alariosis. Wild animals as a source of infection play the leading role in human in-fection. The task of present work is differential diagnosis of larvae at helminthozo-onosis in commercial wild animals (wild boars, wild carnivorous) using method of compressor trichinelloscopy and peptolysis. Methods of compressor trichinel-loscopy and peptolysis of muscle tissue of animals by applying artificial gastric juice can be used at veterinary-sanitary laboratories in markets and meat-processing plants for diagnostics of trichinellosis and alariosis. Veterinary-sanitary expertise of meat from game and commercial hunting is aimed at detection of sources of infection and prevention of helminth zoonosis in human. This expertise should be conducted using a complex method: compressor trichinelloscopy and peptolysis using artificial gastric juice (peptolytic artificial gastric juice). This method contains the description and differentiation of larvae (dangerous to human) released from meat of commercial wild animals using this technique. Practical ap-plication of veterinary-sanitary expertise of meat and meat products from hunter's trophies allows to prevent infection of human and domestic animals with danger-ous zoonosis
151-156 204
Abstract

The article contains the information on using of bibliometric parameters in order to estimate the contribution of research workers to domestic parasitological science. The data have been obtained from the National Database – Science Citation Index of RF (SCI) – and includes some characteristics of various journals in the field of parasitology. The estimation system of research work contains 27 scientometric parameters, the most frequently used are: number of author’s publications in SCI; number of author’s publications found in lists of literature: citation count in SCI; total citation count; h-index etc. It is important for authors to register the bibliometric indicators of publications which they send their articles to.

157-162 163
Abstract

Based on knowledge of epiphytotic process in meloidogynosis the detailed recommendations for protection of soiling crops in bed and industrial greenhouses are provided. Symptoms and injuriousness in meloidogynosis of soiling crops growing in protected ground are presented. A schedule of epiphytotic process is provided and a schedule-based system of protection measures is suggested which includes quarantine measures, management methods and enables to foresee an
impact on all stages of epiphytotic process. Protection measures carried out in bed greenhouses are based on using clean substrates, in industrial greenhouses – on using healthy sprouts, ensuring substrate level control, applying resistant varieties and hybrids, ravaging of weeds. When a nidus has been detected the following measures should be conducted to eliminate this nidus: to localize invasion and increase biological resistance of plants by all plying ameliorants (plant growth
regulating agents), to disinfect ground using biogenic nematicides



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ISSN 1998-8435 (Print)
ISSN 2541-7843 (Online)