Методика количественного определения фенбендазола и его метаболитов в органах и тканях животных методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

Цель исследований: разработать методику количественного определения фенбендазола (ФБЗ) и его метаболитов в органах и тканях овец методом высокоэффективной жидкостной хроматографии с тандемным массспектрометрическим детектированием (ВЭЖХ-МС/МС).

Материалы и методы. Валидацию методики определения ФБЗ и его метаболитов после введения супрамолекулярного комплекса фенбендазола выполняли в соответствии с международными руководствами по показателям: линейность, степень извлечения, специфичность, прецизионность, правильность (точность), пределы количественного и качественного определения.

Результаты и обсуждение. Адаптирован метод количественного анализа ФБЗ и его метаболитов сульфона и сульфоксида в организме овец после введения супрамолекулярного комплекса. Предложенная методика имеет линейную зависимость (R > 0,99) в диапазоне 5–1000 нг/г и показала хорошую воспроизводимость и правильность.

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