vniigisРоссийский паразитологический журналRussian Journal of Parasitology1998-84352541-7843ВНИИП – филиал ФГБНУ ФНЦ ВИЭВ РАН10.31016/1998-8435-2021-15-4-43-50vniigis-826Research ArticleФАРМАКОЛОГИЯ, ТОКСИКОЛОГИЯPHARMACOLOGY, TOXICOLOGYМетодика количественного определения фенбендазола и его метаболитов в органах и тканях животных методом высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированиемMethodology for the quantitative determination of fenbendazole and its metabolites in organs and tissues of animals by high performance liquid chromatography with tandem mass spectrometry detectionhttps://orcid.org/0000-0001-8364-5055ВарламоваА. И.VarlamovaA. I.

Анастасия Ивановна Варламовая, кандидат ветеринарных наук

117218, Москва, ул. Б. Черемушкинская, 28

Anastasiya I. Varlamova, Candidate of Veterinary Sciences

117218, Moscow, B. Cheremushkinskaya st., 28

arsphoeb@mail.ruhttps://orcid.org/0000-0003-4902-5666АбрамовВ. Е.AbramovV. E.

Владислав Евгеньевич Абрамов, доктор ветеринарных наук, профессор

117218, Москва, ул. Б. Черемушкинская, 28

 

Vladislav E. Abramov, Doctor of Veterinary Sciences, Professor

117218, Moscow, B. Cheremushkinskaya st., 28

53.net@mail.ruhttps://orcid.org/0000-0001-5165-0706АрхиповИ. А.ArkhipovI. A.

Иван Алексеевич Архипов, доктор ветеринарных наук, профессор

117218, Москва, ул. Б. Черемушкинская, 28

Ivan A. Arkhipov, Doctor of Veterinary Sciences, Professor

117218, Moscow, B. Cheremushkinskaya st., 28

arkhipovhelm@mail.ruВсероссийский научно-исследовательский институт фундаментальной и прикладной паразитологии животных и растений – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр – Всероссийский научно-исследовательский институт экспериментальной ветеринарии имени К. И. Скрябина и Я. Р. Коваленко Российской академии наук»РоссияAll-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”Russian Federation2021191220211544350Copyright © Варламова А.И., Абрамов В.Е., Архипов И.А., 20212021Варламова А.И., Абрамов В.Е., Архипов И.А.Varlamova A.I., Abramov V.E., Arkhipov I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vniigis.elpub.ru/jour/article/view/826Цель исследований

Цель исследований: разработать методику количественного определения фенбендазола (ФБЗ) и его метаболитов в органах и тканях овец методом высокоэффективной жидкостной хроматографии с тандемным массспектрометрическим детектированием (ВЭЖХ-МС/МС).

Материалы и методы

Материалы и методы. Валидацию методики определения ФБЗ и его метаболитов после введения супрамолекулярного комплекса фенбендазола выполняли в соответствии с международными руководствами по показателям: линейность, степень извлечения, специфичность, прецизионность, правильность (точность), пределы количественного и качественного определения.

Результаты и обсуждение

Результаты и обсуждение. Адаптирован метод количественного анализа ФБЗ и его метаболитов сульфона и сульфоксида в организме овец после введения супрамолекулярного комплекса. Предложенная методика имеет линейную зависимость (R > 0,99) в диапазоне 5–1000 нг/г и показала хорошую воспроизводимость и правильность.

The purpose of the research is to develop a methodology for the quantitative determination of fenbendazole (FBZ) and its metabolites in organs and tissues of sheep by high performance liquid chromatography tandem mass-spectrometry (HPLC-MS/MS).

Materials and methods

Materials and methods. The method for determining of FBZ and its metabolites after the administration of the supramolecular complex of fenbendazole was validated in accordance with international guidelines for the following indicators: linearity, recovery, specificity, precision, accuracy, limits of quantitative and qualitative determination.

Results and discussion

Results and discussion. The method of quantitative analysis of FBZ and its metabolites sulfone and sulfoxide in the body of sheep after the administration of the supramolecular complex has been adapted. The proposed method has a linear relationship (R > 0.99) in the range of 5–1000 ng / g and has shown a good reproducibility and accuracy.

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The authors declare that there are no conflicts of interest present.