OBTAINING IMMUNO REAGENTS FOR SEROLOGICAL DETERMINATION OF DITYLENCHOSIS AGENTS IN AGRICULTURAL AND ORNAMENTAL PLANTS
https://doi.org/10.31016/1998-8435-2019-13-2-89-94
Abstract
The purpose of the research is obtaining immuno reagents for determination of two types of parasitic nematodes Ditylenchus destructor and D. dipsaci (antigens and antiserums for them).
Materials and methods. Three immunizations of laboratory animals (white scrub mice) have been carried out at a dose of 20 mcg of the target protein per animal at 7–10 day intervals. The efficient titer of the antiserums obtained was described by the Dot-ELISA method using secondary goat anti-mouse immunoglobulins conjugated with alkaline phosphatase. For measuring a specificity of the obtained immuno reagents, an ELISA plate in an indirect format was used.
Results and discussion. The antiserums obtained have a specificity for two types of nematodes D. destructor and D. dipsaci, and for one non-parasitic nematode Rhabditis sp. The specificity of the obtained antiserums ranges from 82 to 97%. The sensitivity of antiserums to homogenates of stem nematodes D. destructor and D. dipsaci of plants at the antiserum dilution 1 : 3000 is 1 ng and 50 ng accordingly.
About the Authors
K. O. Butenko
All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants – a branch of Federal State Budgetary Institution of Science "Federal Scientific Center – All-Russian Scientific Research Institute of Experimental Veterinary Medicine named after K. I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences"
Russian Federation
Russian Federation
28, B. Cheremushkinskaya street, Moscow, Russia, 117218.
A. A. Shesteperov
All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants – a branch of Federal State Budgetary Institution of Science "Federal Scientific Center – All-Russian Scientific Research Institute of Experimental Veterinary Medicine named after K. I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences"
Russian Federation
28, B. Cheremushkinskaya street, Moscow, Russia, 117218.
Russian Federation
28, B. Cheremushkinskaya street, Moscow, Russia, 117218.
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Review
For citations:
Butenko K.O., Shesteperov A.A. OBTAINING IMMUNO REAGENTS FOR SEROLOGICAL DETERMINATION OF DITYLENCHOSIS AGENTS IN AGRICULTURAL AND ORNAMENTAL PLANTS. Russian Journal of Parasitology. 2019;13(2):89-94. (In Russ.) https://doi.org/10.31016/1998-8435-2019-13-2-89-94
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