Molecular identification of Giardia duodenalis isolates from dogs in Moscow (Russia)
https://doi.org/10.31016/1998-8435-2026-20-1-73-81
Abstract
The purpose of the research is to genetically characterize Giardia duodenalis isolates from dogs in the city of Moscow (Russia).
Materials and methods. A total of 423 fecal samples from dogs were investigated for G. duodenalis cysts in 2025. Twentyfive isolates were detected, 19 of which were characterized. Genetic analysis was performed based on the β-giardin locus which is 569–581 base pairs long. The obtained sequences were compared with reference sequences in the GenBank database.
Results and discussion. In the present study, for the first time, genotyping of G. duodenalis isolates obtained from domestic dogs in Moscow (Russia) was carried out. Assemblage C was identified in 15.8% of dogs (n = 3/19), assemblage D in 68.4% of dogs (n = 13/19) and three isolates were found to be mixed C and D in 15.8% (n = 3/19). The study revealed the presence of dog-specific assemblages of G. duodenalis that are not zoonotic. However, it is possible that dogs may have other Giardia assemblages, so further studies with a larger number of isolates are needed.
About the Authors
O. P. Kurnosova
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV»
Russian Federation
Russian Federation
Kurnosova Olga P., Ph. D., Senior Researcher, Laboratory of Parasitic Zoonoses
Researcher ID: U-9779-2018
Scopus ID: 23473657000
Moscow
V. S. Zaitsev
Upper Volga State Agro-Biotechnological Univerity; Veterinary laboratory "Zaitsev Plus"
Russian Federation
Russian Federation
Zaitsev Valery S., Ph. D. Associate Professor
Ivanovo
Moscow
O. A. Panova
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV»
Russian Federation
Russian Federation
Panova Olga A., Ph. D., Head of the Laboratory of Biology and Biological Foundations of Prevention
Researcher ID: I-6971-2018
Scopus ID: 57189098000
Moscow
M. V. Arisov
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV»
Russian Federation
Russian Federation
Arisov Michael V., Doctor of Veterinary Sciences, Professor of the Russian Academy of Sciences, Head of the Laboratory of Ectoparasitosis, Director of the VNIIP – FSC VIEV
Researcher ID: B-7834-2018
Scopus ID: 57207942094.
Moscow
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Kurnosova O.P., Zaitsev V.S., Panova O.A., Arisov M.V. Molecular identification of Giardia duodenalis isolates from dogs in Moscow (Russia). Russian Journal of Parasitology. 2026;20(1):73-81. (In Russ.) https://doi.org/10.31016/1998-8435-2026-20-1-73-81
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