Материалы и методы

vniigis

Российский паразитологический журнал

Russian Journal of Parasitology

1998-84352541-7843

ВНИИП – филиал ФГБНУ ФНЦ ВИЭВ РАН

10.31016/1998-8435-2026-20-1-73-81

vniigis-1426

Research Article

БИОХИМИЯ, БИОТЕХНОЛОГИЯ И ДИАГНОСТИКА

BIOCHEMISTRY, BIOTECHNOLOGY AND DIAGNOSTICS

Молекулярная идентификация изолятов Giardia duodenalis, выделенных от собак в г. Москве (Россия)

Molecular identification of Giardia duodenalis isolates from dogs in Moscow (Russia)

https://orcid.org/0000-0002-3248-8931

Курносова

О. П.

Kurnosova

O. P.

Курносова Ольга Петровна, кандидат биологических наук, старший научный сотрудник лаборатории паразитарных зоонозов

Researcher ID: U-9779-2018

Scopus ID: 23473657000

Москва

Kurnosova Olga P., Ph. D., Senior Researcher, Laboratory of Parasitic Zoonoses

Researcher ID: U-9779-2018

Scopus ID: 23473657000

Moscow

kurnosova@vniigis.ru

https://orcid.org/0009-0003-4996-1267

Зайцев

В. С.

Zaitsev

V. S.

Зайцев Валерий Сергеевич, кандидат биологических наук

Иваново

Москва

Zaitsev Valery S., Ph. D. Associate Professor

Ivanovo

Moscow

vetlabplus@gmail.com

https://orcid.org/0000-0001-9254-0167

Панова

О. А.

Panova

O. A.

Панова Ольга Александровна, кандидат биологических наук, заведующая лабораторией биологии и биологических основ профилактики

Researcher ID: I-6971-2018

Scopus ID: 57189098000

Москва

Panova Olga A., Ph. D., Head of the Laboratory of Biology and Biological Foundations of Prevention

Researcher ID: I-6971-2018

Scopus ID: 57189098000

Moscow

panova@vniigis.ru

https://orcid.org/0000-0002-2103-8468

Арисов

М. В.

Arisov

M. V.

Арисов Михаил Владимирович, доктор ветеринарных наук, профессор РАН, заведующий лабораторией эктопаразитозов, руководитель филиала ВНИИП – фил. ФГБНУ ФНЦ ВИЭВ РАН

Researcher ID: B-7834-2018

Scopus ID: 57207942094

Москва

Arisov Michael V., Doctor of Veterinary Sciences, Professor of the Russian Academy of Sciences, Head of the Laboratory of Ectoparasitosis, Director of the VNIIP – FSC VIEV

Researcher ID: B-7834-2018

Scopus ID: 57207942094.

Moscow

director@vniigis.ru

Всероссийский научно-исследовательский институт фундаментальной и прикладной паразитологии животных и растений – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр – Всероссийский научно-исследовательский институт экспериментальной ветеринарии имени К. И. Скрябина и Я. Р. Коваленко Российской академии наук» (ВНИИП – фил. ФГБНУ ФНЦ ВИЭВ РАН)РоссияAll-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV»Russian Federation

Верхневолжский государственный агробиотехнологический университет; Ветеринарная лаборатория «Зайцев Плюс»РоссияUpper Volga State Agro-Biotechnological Univerity; Veterinary laboratory "Zaitsev Plus"Russian Federation

2026

26032026

2017381

2026

Курносова О.П., Зайцев В.С., Панова О.А., Арисов М.В.

Kurnosova O.P., Zaitsev V.S., Panova O.A., Arisov M.V.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vniigis.elpub.ru/jour/article/view/1426

Цель исследований – генетическая характеристика изолятов Giardia duodenalis, выделенных от собак в городе Москве (Россия).

Материалы и методы

Материалы и методы. Всего исследовано 423 пробы фекалий собак на наличие цист G. duodenalis в 2025 г. Было выделено 25 изолятов, 19 из которых были охарактеризованы. Генетический анализ проводили на основе локуса β-гиардин длиной 569–581 пар оснований. Полученные последовательности сравнивали с референсными последовательностями в базе данных GenBank.

Результаты и обсуждение

Результаты и обсуждение. В настоящем исследовании впервые проведено генотипирование изолятов G. duodenalis, полученных от домашних собак в Москве. Группа C была выявлена у 15,8% собак (n = 3/19), группа D — у 68,4% собак (n = 13/19), а три изолята оказались смешанными группами C и D у 15,8% (n = 3/19). Исследование выявило наличие специфичных для собак групп G. duodenalis, не являющихся зоонозными. Однако возможно, что у собак могут быть и другие группы Giardia, поэтому необходимы дальнейшие исследования с большим числом изолятов.

The purpose of the research is to genetically characterize Giardia duodenalis isolates from dogs in the city of Moscow (Russia).

Materials and methods

Materials and methods. A total of 423 fecal samples from dogs were investigated for G. duodenalis cysts in 2025. Twentyfive isolates were detected, 19 of which were characterized. Genetic analysis was performed based on the β-giardin locus which is 569–581 base pairs long. The obtained sequences were compared with reference sequences in the GenBank database.

Results and discussion

Results and discussion. In the present study, for the first time, genotyping of G. duodenalis isolates obtained from domestic dogs in Moscow (Russia) was carried out. Assemblage C was identified in 15.8% of dogs (n = 3/19), assemblage D in 68.4% of dogs (n = 13/19) and three isolates were found to be mixed C and D in 15.8% (n = 3/19). The study revealed the presence of dog-specific assemblages of G. duodenalis that are not zoonotic. However, it is possible that dogs may have other Giardia assemblages, so further studies with a larger number of isolates are needed.

Giardia duodenalisгруппыгенотипированиесобакизоонозная инвазия

Giardia duodenalisassemblagesgenotypingdogszoonotic infection

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The authors declare that there are no conflicts of interest present.