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Diagnostic methods for canine babesiosis

https://doi.org/10.31016/1998-8435-2026-20-1-63-72

Abstract

The purpose of the research is to analyze current diagnostic methods for canine babesiosis and differentiate it from other blood-borne diseases.

Materials and methods. The study was based on long-term literature data, which was analyzed and summarized, highlighting methods for the intravital diagnosis of canine babesiosis.

Results and discussion. Canine babesiosis is a highly prevalent, transmissible hemoprotozoan disease transmitted by ixodid ticks and found worldwide. In dogs, the primary vectors of babesiosis are ticks of the genera Rhipicephalus, Dermatocentor, and Haemaphysalis. Babesiosis is caused by single-celled protozoa of round, oval, pear-shaped, or amoeboid shapes. These organisms are transmitted by tick bites. They parasitize within red blood cells (a single red blood cell can contain from 1 to 32 individuals), blood plasma, neutrophils, and can also be found in parenchymal organs. The most common complications include hemolytic anemia, thrombocytopenia, renal and hepatic failure, and exacerbation of chronic diseases. The disease can be acute, chronic, or subclinical. The incubation period ranges from 3 to 21 days. With the subclinical form, periodic exacerbations are possible. Symptoms may depend on the severity of the disease. The most common symptoms in the acute stage include fever, lethargy, pale or icteric mucous membranes, dark urine, and anorexia. Blood tests reveal splenomegaly and hepatomegaly, and anemia, thrombocytopenia, and monocytosis are noted. Blood smear microscopy, PCR testing, and ELISA diagnostics are used to confirm the disease.

About the Author

E. S. Belomyttseva
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"
Russian Federation

Belomyttseva Evgeniya S., Junior Researcher

Scopus ID: 57222568712



References

1. Belomyttseva E.S., Safiullin R.T. The effectiveness of the drug Diprocarb for babesiosis in carnivores from Moscow and the Moscow region. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2017; 11 (2): 183-187. (In Russ.)

2. Vaden Shelley L., Noll Joyce S., Smith Jr. Francis W. K., Tilley Larry P. Complete Guide to Laboratory and Instrumental Research in Dogs and Cats. Moscow: Aquarium-Print, 2014; 138-140. (In Russ.)

3. Niemand Hans G., Suter Peter F. Diseases of Dogs. Moscow: Aquarium, 2004; 261-262. (In Russ.)

4. Ramsey Ya. Tennant B. Infectious Diseases of Dogs and Cats. M.: Aquarium, 2019; 86-87. (In Russ.)

5. Beugnet F., Moreau Y. Babesiosis. Revue scientifique et technique (International Office of Epizootics). 2015; 34: 627–639. https://doi.org/10.20506/rst.34.2.2385

6. Birkenheuer A. J., Levy M. G., Breitschwerdt E. B. Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (Asian genotype) and B. canis DNA in canine blood samples. Journal of clinical microbiology. 2003; 41: 4172–4177. https://doi.org/10.1128/JCM.41.9.4172-4177.2003

7. Bohm M., Leisewitz A. L., Thompson P. N., Schoeman J. P. Capillary and venous Babesia canis rossi parasitaemias and their association with outcome of infection and circulatory compromise. Veterinary parasitology. 2006; 141: 18–29. https://doi.org/10.1016/j.vetpar.2006.05.002

8. Bone R. C., Balk R. A., Cerra F. B., Dellinger R. P., Fein A. M., Knaus W. A., Schein R. M., Sibbald W. J. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine. 1992; 101: 1644-1655. https://doi.org/10.1378/chest.101.6.1644

9. Boozer A.L., Macintire D.K. Canine babesiosis. Veterinary Clinics of North America: Small animal

10. practice. 2003; 33: 885–904. https://doi.org/10.1016/s0195-5616(03)00039-1

11. Do Thom, Phoosangwalthong Pornkamol, Ketsarin Kamyingkird, Chanya Kengradomkij, Wissanuwat Chimnoi, Tawin Inpankaew. Molecular detection of tick-borne pathogens in stray dogs and Rhipicephalus sanguineus sensulato ticks from Bangkok Thailand. Pathogens. 2021; 10 (5): 561.

12. https://doi.org/10.3390/pathogens10050561

13. Duarte S. C., Linhares G. F. C., Romanowsky T. N., Neto O. J., Borges L. M. F. Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and by PCR assay. Veterinary parasitology. 2008; 152: 16–20. https://doi.org/10.1016/j.vetpar.2007.12.013

14. Efstratiou A., Karanis G., Karanis P. Tick-borne pathogens and diseases in Greece. Microorganisms. 2021; 9 (8): 1732. https://doi.org/10.3390/microorganisms9081732

15. Eichenberger R. M., Ramakrishnan C., Russo G., Deplazes P., Hehl A. B. Genome-wide analysis of

16. gene expression and protein secretion of Babesiacanis during virulent infection identifies potential pathogenicity factors. Scientific reports. 2017; 7 (1): 3357. https://doi.org/10.1038/s41598-017-03445-x

17. Eslahi A. V., Olfatifar M., Zaki L., Pirestani M., Sotoodeh S., Farahvash M. A., Maleki A., Badri M. The worldwide prevalence of intestinal helminthic parasites among food handlers: A systematic review and meta-analysis. Food Control. 2023; 148 (2): 109658. https://doi.org/10.1016/j.foodcont.2023.109658

18. Galon E. M., Zafar I., Ji S., Li H., Ma Z., Xuan X. Molecular reports of ruminant Babesia in southeast Asia. Pathogens. 2022; 11 (8): 915. https://doi.org/10.3390/pathogens11080915

19. Garcia K., Weakley M., Do T., Mir S. Current and future molecular diagnostics of tick-borne diseases in cattle. Veterinary sciences. 2022; 9 (5): 241. https://doi.org/10.3390/vetsci9050241

20. Hamel D., Rohrig E., Pfister K. Canine vectorborne disease in travelled dogs in Germany-a retrospective evaluation of laboratory data from the years 2004–2008. Veterinary parasitology. 2011; 181 (1): 31-36. https://doi.org/10.1016/j.vetpar.2011.04.020

21. Ikadai H., Tanaka H., Shibahara N., Matsuu A., Uechi M., Itoh N., Oshiro S., Kudo N., Igarashi I.,

22. Oyamada T. Molecular Evidence of Infections with Babesia gibsoni Parasites in Japan and Evaluation of the Diagnostic Potential of a Loop-Mediated Isothermal Amplification Method. Journal of clinical microbiology. 2004; 42 (6): 2465-2469. https://doi.org/10.1128/JCM.42.6.2465-2469.2004

23. Irwin P. J., Hutchinson G. W. Clinical and pathological findings of Babesia infection in dogs. Australian veterinary journal. 1991; 68 (6): 204209. https://doi.org/10.1111/j.1751-0813.1991.tb03194.x

24. Irwin P. J. Canine babesiosis: from molecular taxonomy to control. Parasites & vectors. 2009; 2 Suppl 1(Suppl 1): S4. https://doi.org/10.1186/1756-3305-2-S1-S4

25. Koster L. S., Lobetti R. G., Kelly P. Canine babesiosis: a perspective on clinical complications, biomarkers, and treatment. Veterinary medicine: Research reports. 2015; 6: 119-128. https://doi.org/10.2147/VMRR.S60431

26. Maegraith B., Gilles H. M., Devakul K. Pathological processes in Babesia canis infections.Zeitschrift für Tropenmedizin und parasitologie. 1957; 8 (4): 485-514.

27. Mehlhorn H., Schein E. The Piroplasms: Life Cycle and Sexual Stages. Advanсes in parasitology.

28. ; 23: 37-103. https://doi.org/10.1016/s0065-308x(08)60285-7

29. Miro G., Checa R., Paparini A., Ortega N., Gonzalez, Fraga J. L., Gofton A., Bartolome A., Montoya A., Galvez R., Mayo P. P., Irwin P.: Theileria annae (syn. Babesia microti-like) infection in dogs in NW Spain detected using direct and indirect diagnostic techniques: clinical report of 75 cases. Parasites & vectors. 2015; 8: 217. https://doi.org/10.1186/s13071-015-0825-2

30. Penzhorn B. L. Don't let sleeping dogs lie: Unravelling the identity and taxonomy of Babesia canis, Babesia rossi and Babesia vogeli. Parasites & vectors. 2020; 13 (1): 184. https://doi.org/10.1186/s13071-020-04062-w

31. Purvis D., Kirby R. Systemic inflammatory response syndrome: septic shock. The Veterinary clinics of North America. Small animal practice. 1994; 24 (6): 1225-47. https://doi.org/10.1016/s0195-5616(94)50136-0

32. Schetters T., Moubri K., Precigout E., Kleuskens J., Scholtes N. C., Gorenflot A. Different Babesia canis isolates, different diseases. Parasitology. 1997; 115 (Pt 5): 485-93. https://doi.org/10.1017/s0031182097001686

33. Schnittger L., Rodriguez A. E., Florin-Christensen M., Morrison D. A. Babesia: a world emerging. Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics of infectious diseases. 2012; 12 (8): 1788-809. https://doi.org/10.1016/j.meegid.2012.07.004

34. Schoeman J. P. Canine babesiosis. The Onderstepoort journal of veterinary research. 2009; 76 (1): 59-66.

35. Uilenberg G. Babesia – A historical overview. Veterinary parasitology. 2006; 138 (1-2): 3-10. https://doi.org/10.1016/j.vetpar.2006.01.035

36. Vannier E. G., Diuk-Wasser M. A., Mamoun C. B., Krause P. J. Babesiosis. Infectious disease clinics of North America. 2015; 29 (2): 357-370. https://doi.org/10.1016/j.idc.2015.02.008

37. Vercammen F., De Deken R., Maes L. Duration of protective immunity in experimental canine

38. babesiosis after homologous and heterologous challenge. Veterinary parasitology. 1997; 68 (1-2): 51-55. https://doi.org/10.1016/s0304-4017(96)01063-1

39. Wang C., Ahluwalia S. K., Li Y., Gao D., Poudel A., Chowdhury E., Boudreaux M. K., Kaltenboeck B.

40. Frequency and therapy monitoring of canine Babesia spp. infection by high resolution melting curve quantitative FRET-PCR. Veterinary parasitology. 2010; 168 (1-2): 11-18. https://doi.org/10.1016/j.vetpar.2009.10.015

41. Welzl C., Leisewitz A. L., Jacobson L. S., Vaughan Scott T., Myburgh E. Systemic inflammatory response syndrome and multiple-organ damage/dysfunction in complicated canine babesiosis. Journal of the South African Veterinary Association. 2001; 72 (3): 158-162. https://doi.org/10.4102/jsava.v72i3.640


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Belomyttseva E.S. Diagnostic methods for canine babesiosis. Russian Journal of Parasitology. 2026;20(1):63-72. (In Russ.) https://doi.org/10.31016/1998-8435-2026-20-1-63-72

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ISSN 1998-8435 (Print)
ISSN 2541-7843 (Online)