Evaluation of the Subchronic Toxicity of the Complex Preparation for Dogs and Cats "Inspector Kvadro" for Cutaneous Use

The purpose of the research is to assess the subchronic toxicity of the complex drug for dogs and cats “Inspector Kvadro” for cutaneous use.

Materials and methods. The experiment was carried out on 40 male rats with an initial body weight of 220–240 g. The animals were divided into 4 equivalent groups of 10 rats each. The drug was applied daily for 7 days on a clipped skin in the back at doses of 1080; 540 and 216 mg/kg. During the experiment, we observed the general condition and behavior of animals, possible death, intake of food and water, visible physiological functions, the condition of the coat and skin at the site of application of the drug in order to evaluate the irritating effect. On the first day and 10 days after the last use of the drug, the animals were euthanized and blood samples were taken to determine hematological and biochemical parameters, a macroscopic examination of the organs was carried out, and the functional state of the central nervous system was evaluated.

Results and discussion. Daily application on the skin of the drug in test doses did not lead to a change in the general condition and behavior of rats. Experienced animals normally took food and water, adequately responded to external stimuli. However, the recorded changes in some indicators indicate damage to the hepatocytes and indicate insufficient liver function. Given the increased relative liver mass of the animals of the first experimental group and the change in biochemical parameters, we can conclude that the drug has a pronounced hepatotoxic effect at a dose of 1080 mg/kg for cutaneous use for 7 days. Accordingly, the dose of 1080 mg/kg is toxic, the doses of 540 and 216 mg/kg are threshold. 

1. Arisov M. V., Stepanov V. A., Smirnova E. S. Pharmaco-toxicological evaluation of a comprehensive antiparasitic drug for dogs and cats. Rossiyskiy veterinarnyy zhurnal. Melkiye domashniye i dikiye zhivotnyye = Russian Veterinary Journal. Small domestic and wild animals. 2014; 4: 36–39. (In Rus.)

2. Arisov M. V., Stepanova I. A., Koshkarev E. A., Arisova G. B. The effectiveness of the drug “Neoteric Protecto 4” against ixodidosis and other animal acarosis. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2018; 12(2): 68–74. (In Rus.)

3. Indyuhova E. N., Arisov M. V., Arisova G. B., Stepanova I. A. Toxicological evaluation of the complex insecticide and acaricidal preparation “Neoterica Protecto 12”. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2018; 12(3): 60–61. (In Rus.)

4. Guidance on the experimental (preclinical) study of new pharmacological substances / Under the general editorship of Corresponding Member of RAMS, Professor Khabriev R. U. M.: Medicine, 2005; 832. (In Rus.)

5. Stepanov V. A., Arisov M. V., Smirnova E. S. Toxicological evaluation and insecticide and acaricidal efficacy of RolfClub 3D spray for dogs and RolfClub 3D spray for cats. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2014; 3: 112–117. (In Rus.)