Detection and Morphology of the Larval Stages of the Trematode Gymnophallus Rebecqui (Bartoli, 1983) (Trematoda: Gymnophallidae) in Bivalve Mollusks off the Black Sea Coast of Crimea
https://doi.org/10.31016/1998-8435-2019-13-3-39-46
Abstract
The purpose of the research description of larvae Gymnophallus rebecqui (Bartoli, 1983) morphology from bivalve mollusks Abra segmentum Recluz, 1843 and Cerastoderma glaucum Poiret, 1789 in the Black Sea; to study seasonal abundance of G. rebecqui in the Black Sea.
Materials and methods. During 2011–2012 years, 875 samples of bivalves A. segmentum and 440 samples of C. glaucum were investigated from two biotopes Sevastopol water area. The samples were selected every month. All mollusks tissues were investigated for parasites with a compressor method over binocular at increase × 98. The detected cercarias and metacercarias were fixed for life and dyed with acetous carmine, and after dehydration in a series of alcohol and clarification in clove oil were placed in Canada balsam. One of the generally accepted systems of trematodes measuring was used in the work. Measurings were made on microscope XY-B2 with с a film camera at increase × 1000. The images were made in redactor of vector graphics Inkscape 0.48.2-1.
Results and discussion. For the first time, at the estuary of the river Chernaya and in the Black Sea, larvae of trematodes G. rebecqui have been found in mollusks A. segmentum and C. glaucum. A. segmentum has appeared to be not only the second intermediate host for such trematode in the studied ecosystem but also parthenitas G. rebecqui were detected in it. According to the main diagnostic and morphological features, the analyzed larvae of trematodes were identified as G. rebecqui: the Y-shaped secretory cyst without diverticula in cercarias, and the absence of the ventral hole and of accumulation of prostatic cells over the ventral sucker at the metacercaria stage. The morphometric features of the cercarias and metacercarias of trematodes G. rebecqui, which were studied by us, are within the boundaries known for larvae of this type parasitizing in mollusks A. segmentum and C. glaucum off the coasts of France, Great Britain and the Black Sea. An infection peak of mollusks C. glaucum with metacercarias G. rebecqui was observed in late summer and early autumn in both regions.
About the Author
Yu. V. Belousova
A. O. Kovalevsky Institute of Marine Biological Researches of the Russian Academy of Sciences
Russian Federation
299011, Sevastopol, Nakhimova prospect, 2
Russian Federation
299011, Sevastopol, Nakhimova prospect, 2
References
1. Bykhovskaya–Pavlovskaya I. Е. Parasitologic study of fish. L.: Science, 1969; 108. (In Russ.)
2. Prokofiev V. V., Galaktionov K. V. Strategies of trematode cercarias’ searching behavior. Works of the Institute of Zoology of the Russian Academy of Sciences. 2009; 313 (3): 265–284. (In Russ.)
3. Chubrik G. K. Parasitological studies of mollusks of the eastern Murman and the White Sea coastal region. Works of problematic and topical meetings of the Institute of Zoology of the Russian Academy of Sciences. VII meeting on parasitological issues. 1954; 4: 128–133. (In Russ.)
4. Bartoli P. Gymnophallus rebecqui n. sp. (syn. Parvatrema sp. l., J. Rebecq, 1964) (Digenea: Gymnophallidae) parasite intestinal d’anatidés de Camargue (France). Annales de parasitologie humaine et compare. 1983; 58: 211–225.
5. Bowers E. A. The metacercariae of sibling species of Meiogymnophallus, including M. rebecqui comb. nov. (Digenea: Gymnophallidae), and their effects on closely related Cerastoderma host species (Mollusca: Bivalvia). Parasitology Research. 1996; 82(6): 505–510.
6. Campbell D. The life cycle of Gymnophallus rebecqui (Digenea: Gymnophallidae) and the response of the bivalve Abra tenuis to its metacercari. J. mar. biol. Ass. U.K. 1985; 65: 589–601.
7. Cremonte F., Gilardoni C., Pina S., Rodrigues P., Ituarte C. Revision of the Family Gymnophallidae Odner, 1905 (Digenea) based on morphological and molecular data. Parasitology International. 2015; 64: 202–210.
8. Dmitrieva E. V., Lyakh A. M., Kornyychuk Yu. M., Polyakova T. A., Popyuk M. P. (2015) IMBR Collection of marine parasites: the collection of marine parasites maintained by the Institute of Marine Biological Research. www.marineparasites. org. (Accessed at Nov 26, 2016.)
9. Galaktionov K. V., Irwin S. W. B., Saville D. H. One of the most complex life-cycles among trematodes: a description of Parvatrema margaritense (Ching, 1982) n. comb. (Gymnophallidae) possessing parthenogenetic metacercariae. Parasitology. 2006; 132: 733–746.
10. Galaktionov K. V. A description of the parthenogenetic metacercaria and cercaria of Cercaria falsicingulae I larva nov. (Digenea: Gymnophallidae) from the snails Falsicingula spp. (Gastropoda), with speculation on an unusual lifecycle. Syst. Parasitol., 2007; 68: 137–146.
11. Gibson D. I., Jones A., Bray A. Keys to the Trematoda Eds. Wallingford: CABI Publ., 2002; Vol. 1.
12. Kostadinova A. Dicrogaster perpusilla Loos, 1902 sensu Sarabeev, Balbuena (Digenea: Haploporidae): a note of caution. Syst. Parasitol. 2009; 73: 141–150.
13. Lebour M. V. Trematodes of Northumberland coast. Transactions of the Natural History Society of Northumberland, Durham and Newcastleupon 1908. Tyne, 3. P. 28–45.
14. Pekkarinen M. Development of the cercaria of Lacunovermis macomae (Trematoda: Gymnophallidae) to the metacercaria in brackish-water Macoma balthica (Bivalvia). Ann. Zool. Fennici. 1986; 23: 237–250.
15. Pekkarinen M. The cercaria of Lacunovermis macomae (Lebour, 1908) (Trematoda: Gymnophallidae), and its penetration into the bivalve Macoma balthica (L.) in experimental conditions. Ann. Zool. Fennici. 1987; 24: 101–121.
16. Scholz T. Family Gymnophallidae Odhner, 1905. Keys to Trematoda. Contr.: D. I. Gibson, A. Jones, R. A. Bray. Oxon, U.K.: Cabi Publishing, 2002; 1: 245–251.
17. Stunkard H. W., Uzmann J. R. Studies on digenetic trematodes of the genera Gymnophallus an-d Parvatrema. Biological Bulletin. Marine Biological Laboratory, Woods Hole, Mass., 1958; 115: 276–302.
18. Vilott M. A. On the migrations and metamorphoses of the marine endoparasitic trematodes. Annales and Magazine of Natural History. 1875; 16: 302–304.
Review
For citations:
Belousova Yu.V. Detection and Morphology of the Larval Stages of the Trematode Gymnophallus Rebecqui (Bartoli, 1983) (Trematoda: Gymnophallidae) in Bivalve Mollusks off the Black Sea Coast of Crimea. Russian Journal of Parasitology. 2019;13(3):39-46. (In Russ.) https://doi.org/10.31016/1998-8435-2019-13-3-39-46
Views:
364
Email this article 