Determination of D-Cyphenothrin and Pyriproxyfen residuals in blood serum and eggs of laying hens after their external treatment with an insectoacaricide aqueous emulsion

The purpose of the research is to determine D-cyphenothrin and Pyriproxyfen residuals in blood serum and eggs of laying hens before and after their external treatment with an aqueous emulsion of insectoacaricide D-Cyphenothrin Combo.

Materials and methods. Six SP-789 laying hens were kept individually in cages. Blood samples were taken before treatment and at 6, 12, 24, and 48 hours; eggs were collected before and for 5 days after the treatment that was conducted with a 0.005% aqueous emulsion of D-Cyphenothrin Combo in a fine droplet. The D-Cyphenothrin and Pyriproxyfen concentration in the samples was determined by liquid chromatography/mass spectrometry (LCMS) with a Shimadzu LCMS-8050 GC-MS system and other laboratory equipment. The lower level of quantification (LLOQ) for D-Cyphenothrin was 25.0 ng/mL in blood serum of the chickens and 25.0 ng/g in the egg; and the LLOQ for Pyriproxyfen was 0.5 ng/mL in blood serum and 0.5 ng/g in the egg.

Results and discussion. One chicken was determined to have the Pyriproxyfen concentration above the LLOQ (0.760 ng/mL) in the blood serum 6 hours after treatment. All other samples obtained from SP-789 egg-laying hens showed the concentrations of D-Cyphenothrin and Pyriproxyfen below the LLOQ. Therefore, it can be assumed that the use of eggs was not limited for food purposes after external application of a cyphenothrin and pyriproxyfen drug. Piperonyl butoxide, which is part of D-Cyphenothrin Combo, was not found by the LCMS method due to the peculiarities of its chemical structure. Taking into account the drug composition, the quantitative content of its components, the usage procedure (external only), and the data of regulatory documents, an excess of Piperonyl butoxide in food products is not expected. In view of the above, the use of eggs from chickens after external treatment with a 0.005% aqueous emulsion of the studied drug for food purposes is not limited.

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