Visual and molecular serologic diagnosis of sarcocystosis in cattle

Abstract

The purpose of the research is to provide a comparative assessment of efficacy of different diagnostic methods for sarcocystosis in cattle.
Materials and methods. Seventy-eight bovine carcasses were examined using methods and regulations of veterinary and sanitary examination. Muscles of the esophagus and other parts of the carcass as well as the spleen, lungs, the liver, and kidneys were examined. The compressor research method was used. A total of 156 compressoria from 78 bovine carcasses were analyzed for tissue sarcocystosis. For life-time diagnostics of bovine sarcocystosis, a molecular serologic method (ELISA) was used that was developed based on antibody detection principles.
Results and discussion. Two diagnostic methods, visual inspection and compressor microscopy, identified 13 out of 78 carcasses with Sarcocystis species, which was 16.6%. The number of sarcocystosis animals increased by another 23 cases when studying blood sera from the cattle using ELISA. Thus, we found 36 animals suffering from sarcocystosis out of 78 examined, which was 46%, with tests based on different principles.