Efficiency of sandwich enzyme immunoassay for the diagnosis of larval echinococcus infection in cattle in the Republic of Azerbaijan
https://doi.org/10.31016/1998-8435-2023-17-3-378-385
Abstract
The purpose of the research is to develop a sandwich enzyme-linked immunosorbent assay (ELISA) for laboratory diagnosis of echinococcosis in animals.
Materials and methods. The diagnostic efficiency of the ELISA test system developed by us against cystic echinococcus infection in cattle was studied by comparing the obtained results with the data of a postmortem examination of animal carcasses. The sera from 80 animals delivered to the slaughterhouse from farms of different regions of the Republic of Azerbaijan were tested.
Results and discussion. A positive ELISA test result was obtained in 42 animals, which was 52.5% of the total number of the animals studied. The postmortem diagnosis was confirmed in 32 animals, which was 40.0%. The comparison of the ELISA diagnostic efficiency with the postmortem examination data was 68.75%. Fasciola species were found in the bile ducts in 7 animals with a positive ELISA test response to echinococcosis and a negative diagnosis in the postmortem examination, and Cysticercus tenuicollis was found in one animal.
About the Authors
S. I. Rustamova
Ministry of Agriculture of Azerbaijan, Scientific-Research Veterinary Institute
Azerbaijan
Azerbaijan
Rustamova Siala Ismail, Candidate of Agricultural Sciences, Director
8 per., Boyuk-Shor Highway, Nizami District, AZ1016, Baku
A. A. Sizov
Siberian Federal Scientific Center of Agro-BioTechnologies of the RAS
Russian Federation
Russian Federation
Sizov Alexander A., Leading Researcher
Centralnaya Str., Krasnoobsk Township, Novosibirsk Region, 630501
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Review
For citations:
Rustamova S.I., Sizov A.A. Efficiency of sandwich enzyme immunoassay for the diagnosis of larval echinococcus infection in cattle in the Republic of Azerbaijan. Russian Journal of Parasitology. 2023;17(3):378-385. (In Russ.) https://doi.org/10.31016/1998-8435-2023-17-3-378-385
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