Methodology for the quantitative determination of fenbendazole and its metabolites in organs and tissues of animals by high performance liquid chromatography with tandem mass spectrometry detection
https://doi.org/10.31016/1998-8435-2021-15-4-43-50
Abstract
The purpose of the research is to develop a methodology for the quantitative determination of fenbendazole (FBZ) and its metabolites in organs and tissues of sheep by high performance liquid chromatography tandem mass-spectrometry (HPLC-MS/MS).
Materials and methods. The method for determining of FBZ and its metabolites after the administration of the supramolecular complex of fenbendazole was validated in accordance with international guidelines for the following indicators: linearity, recovery, specificity, precision, accuracy, limits of quantitative and qualitative determination.
Results and discussion. The method of quantitative analysis of FBZ and its metabolites sulfone and sulfoxide in the body of sheep after the administration of the supramolecular complex has been adapted. The proposed method has a linear relationship (R > 0.99) in the range of 5–1000 ng / g and has shown a good reproducibility and accuracy.
About the Authors
A. I. Varlamova
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”
Russian Federation
Russian Federation
Anastasiya I. Varlamova, Candidate of Veterinary Sciences
117218, Moscow, B. Cheremushkinskaya st., 28
V. E. Abramov
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”
Russian Federation
Russian Federation
Vladislav E. Abramov, Doctor of Veterinary Sciences, Professor
117218, Moscow, B. Cheremushkinskaya st., 28
I. A. Arkhipov
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”
Russian Federation
Russian Federation
Ivan A. Arkhipov, Doctor of Veterinary Sciences, Professor
117218, Moscow, B. Cheremushkinskaya st., 28
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Review
For citations:
Varlamova A.I., Abramov V.E., Arkhipov I.A. Methodology for the quantitative determination of fenbendazole and its metabolites in organs and tissues of animals by high performance liquid chromatography with tandem mass spectrometry detection. Russian Journal of Parasitology. 2021;15(4):43-50. (In Russ.) https://doi.org/10.31016/1998-8435-2021-15-4-43-50
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