Study of the acute toxicity of the medicinal product for veterinary use Iverbutan
https://doi.org/10.31016/1998-8435-2021-15-3-76-82
Abstract
The purpose of the research is to study the acute oral toxicity of the medicinal product for veterinary use Iverbutan, intended for the treatment and prevention of arachnoentomoses and nematodoses of poultry.
Materials and methods. The studies were carried out on 30 outbred male rats weighing 210-240 g and 60 mice weighing 18–21 g. The animals were divided into experimental and control groups. The drug was administered once without dilution in the form of the provided solution using an intragastric tube. Doses of 2000, 4000, 6000, 8000 and 10 000 mg/kg were tested on mice, and on rats – 10 000, 8000, 5000, 4000 mg/kg. The animals of the control groups were injected with drinking water. Within 14 days after a single dose of the drug, the physiological state and behavior of animals, possible death, as well as the manifestation of symptoms of intoxication were monitored. The control of the body weight of the animals of the experimental and control groups was carried out on the day of the experiment (before drug administration), as well as on the 1st, 3rd, 7th, 9th and 14th days.
Results and discussion. It was found that after oral administration of iverbutan to experimental animals, the average lethal dose, calculated by the Kerber method, was 5600 mg/kg of body weight in mice and 7000 mg/kg of body weight in rats (hazard class 4 according to GOST 12.1.007-76). The average lethal dose, calculated by the Miller and Tainter method, was 5292.0±1058.6 (4233.4÷6350.6) mg/kg of body weight in mice and 6463.2±1496.9 (4966.3÷7960.1) mg/kg of body weight of rats (hazard class 3 according to GOST 12.1.007-76), which indicates species sensitivity.
About the Authors
E. N. Indyuhova
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”
Russian Federation
Russian Federation
Evgenia N. Indyuhova
28, B. Cheremushkinskaya st., Moscow, 117218
G. B. Arisova
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”
Russian Federation
Russian Federation
Gulnara B. Arisova
28, B. Cheremushkinskaya st., Moscow, 117218
I. P. Belykh
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”
Russian Federation
Russian Federation
Irina P. Belykh
28, B. Cheremushkinskaya st., Moscow, 117218
D. S. Poselov
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”
Russian Federation
Russian Federation
Dmitry S. Poselov
28, B. Cheremushkinskaya st., Moscow, 117218
A. A. Stepanov
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV”
Russian Federation
Russian Federation
Alexey A. Stepanov
28, B. Cheremushkinskaya st., Moscow, 117218
References
1. Arisov M. V., Kozhina A. V., Indyuhova E. N. Modeling the formulation of a combined preparation for parasitosis of birds with the optimization of their metabolism and nonspecific resistance. Materialy XII nauchno-prakticheskoy konferentsii pamyati prof. V. A. Romashova «Sovremennyye problemy obshchey i prikladnoy parazitologii» = Materials of the XII scientific-practical conference in memory of prof. V. A. Romashova "Modern problems of general and applied parasitology". Voronezh, 2018; 92-97. (In Russ.)
2. GOST 12.1.007-76. Harmful substances. Classification and general safety requirements.
3. Directive 2010/63 / EU of the European Parliament and of the Council of the European Union of 22 September 2010 on the protection of animals used for scientific purposes.
4. Emelyanova N. B. Acute oral and cutaneous toxicity of antiparasitic salt briquettes on laboratory animals. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2013; 4: 97-99. (In Russ.)
5. Zashchepkina V. V. Acute oral toxicity of the supramolecular complex of ivermectin. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2020; 14(1): 59-63. DOI: https://doi.org/10.31016/1998-8435-2020-14-1-59-63 (In Russ.)
6. Retskiy M. I., Kaverin N. N., Argunov M. N. Toxicology. Part 1. Textbook for universities. Voronezh, 2006; 55. (In Russ.)
7. Guidelines for conducting preclinical studies of drugs. Part 1. M.: Grif i K, 2012; 944. (In Russ.)
8. Guidelines for experimental (preclinical) study of new pharmacological substances / Under the general editorship of Corresponding Member. RAMS, prof. R. U. Khabrieva M.: Medicine, 2005; 832. (In Russ.)
9. Arisov M. V., Urazaev D. N., Kachanova E. O., Pavlova A. S. General principles of conducting preclinical toxicology studies of antiparasitic drugs for veterinary use. In: IOP Conference Series: Earth and Environmental Science: III International Scientific Conference: AGRITECHIII-2020: Agribusiness, Environmental Engineering and Biotechnologies. Volgograd, Krasnoyarsk, 18-20 июня 2020 года / Krasnoyarsk Science and Technology City Hall of the Russian Union of Scientific and Engineering Associations. Volgograd, Krasnoyarsk: Institute of Physics and IOP Publishing Limited, 2020; 42042. DOI https://doi.org/10.1088/1755-1315/548/4/042042
10. European Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes (ETS 123). Strasbourg, 1986.
11. Guide for the care and use of laboratory animals. National Academy press. Washington, D. C., 1996.
12. Laing R., Gillan V., Devaney E. Ivermectin – old drug, new tricks? Trends in parasitology. 2017; 33(6): 463-472.
13. Sutherland I. H., Campbell W. C. Development, pharmacokinetics and mode of action of ivermectin. Acta Leidensia. 1990; 59(1-2): 161- 168.
14. Woodward K. N. Macrocyclic lactone endectocides. Mammalian Toxicology of Insecticides. 2012; 12. 427-464.
Review
For citations:
Indyuhova E.N., Arisova G.B., Belykh I.P., Poselov D.S., Stepanov A.A. Study of the acute toxicity of the medicinal product for veterinary use Iverbutan. Russian Journal of Parasitology. 2021;15(3):76-82. (In Russ.) https://doi.org/10.31016/1998-8435-2021-15-3-76-82
Views:
429
Email this article 