Evaluation of karyopathic effects and general safety of Trichinella spiralis and Echinococcus multilocularis extracts in mice
https://doi.org/10.31016/1998-8435-2020-14-4-90-98
Abstract
The purpose of the research is assessment of karyopathic effect and general safety of Trichinella spiralis and Echinococcus multilocularis protein extracts in mice.
Materials and methods. To assess the karyotic effect bone marrow cells were isolated and preparations were prepared using a metaphase method (Ford C. E., Hamerton J. L., 1956). Cytological analysis of bone marrow smears was performed to determine the white cell lineage counts. The main hematological indices of mice were determined at hematological analyzer «Abacus JuniorVet» («Diatron», Austria); differential white blood cell count was analyzed using common method. Blood biochemical indices were determined at biochemical analyzer A-15/25 (BioSystems S.A., Spain).
Results and discussion. The intraperitoneal administration of T. spiralis and E. multilocularis protein extracts induced K-mitosis in mice bone marrow cell population. Systemic disorders associated with that cytogenetic effect and general reaction of organism to administration of protein extracts, in particular changes in bone marrow white cell lineage counts and peripheral blood indices were revealed. T. spiralis and E. multilocularis protein products showed some negative effects on liver and kidney function in mice.
About the Authors
E. I. Koveshnikova
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"
Russian Federation
Russian Federation
Elena I. Koveshnikova
28, Bolshaya Cheremushkinskaya st., Moscow, 117218
T. S. Novik
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"
Russian Federation
Russian Federation
Tamara S. Novik
28, Bolshaya Cheremushkinskaya st., Moscow, 117218
L. A. Napisanova
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"
Russian Federation
Russian Federation
Ludmila A. Napisanova
28, Bolshaya Cheremushkinskaya st., Moscow, 117218
S. I. Chukina
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"
Russian Federation
Russian Federation
Svetlana I. Chukina
28, Bolshaya Cheremushkinskaya st., Moscow, 117218
O. V. Rudneva
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant a branch of the Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"
Russian Federation
Russian Federation
Olga V. Rudneva
28, Bolshaya Cheremushkinskaya st., Moscow, 117218
References
1. Bekish V. Ya, Bekish O.-Ya. L. The state of host genome at helminthoses. Vitebsk, 2004. (In Russ.)
2. Bochkov N. P., Demin Yu. S., Luchnik N. V. Classification and methods of accounting for chromosomal aberrations in somatic cells. Genetika = Genetics. 1972; 5: 131–141. (In Russ.)
3. Koveshnikova E. I., Novik T. S., Rudneva O. V., Chukina S. I. Study of the effect of Trichinella of various types and stages of development on mitosis in the host. Meditsinskaya parazitologiya i parazitarnyye bolezni = Medical parasitology and parasitic diseases – in print. (In Russ.)
4. The Order of the Ministry of Health of the USSR No. 755 of 12.08.1977, "Rules for performance of work using experimental animals".
5. The Rules of Laboratory Practice. The Order of the Ministry of Health of the Russian Federation No. 708n, 23.08.2010.
6. The Order of the Ministry of Health of the USSR No. 1045-73, 06.04.1973. “Sanitary rules for design, equipment and maintenance of experimental biological clinics (vivariums)”. (In Russ.)
7. Sivkova T. N. The karyopathic effect of somatic products of Hydatigera on the bone marrow of laboratory animals. Sbornik materialov Regional'noy konf. molodykh uchenykh «Sovremennyye tendentsii razvitiya APK v Severnom Zaural'ye» = Materials of the Regional Conference of Young Scientists "Modern trends in development of Agroindustrial Complex in the Northern Trans-Urals". Tyumen, 2009; 67–69. (In Russ.)
8. Sivkova T. N. The karyopathic effect of Anisakidae larvae products on somatic and germ cells of laboratory rats at per oral administration. Materialy dokladov nauchnoy konferentsii Vserossiyskogo obshchestva gel'mintologov RAN «Teoriya i praktika bor'by s parazitarnymi boleznyami» = Materials of reports of the scientific conference of the All-Russian Society of Helminthologists of the Russian Academy of Sciences "Theory and practice of parasitic disease control". 2009; 10: 366–370. (In Russ.)
9. Sivkova T. N. The karyopathic effect of somatic products of Diphyllobothrium latum on red bone marrow cells. Materialy dokladov Vserossiyskoy nauchno-prakticheskoy konferentsii «Sovremennyye dostizheniya veterinarnoy meditsiny sel'skokhozyaystvennomu proizvodstvu» = Materials of reports of the All-Russian scientificpractical conference "Modern achievements of veterinary medicine in agricultural production". Pos. Persianovsky, 2009; 85–87.
10. Sivkova T. N. Karyopathic and pathomorphological changes caused by parasite metabolism products and the effect on the reproductive function of domestic carnivores: Abstract of thesis for the degree of Doctor of Biological Sciences, 2010.
11. European Convention for Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes (ETS 123). Strasbourg, 1986.
12. Ford C. E., Hamerton J. L. A colchicines, hypotonic citrate squach sequence for mammalian chromosomes. Stain Technol. 1956; 31 (6): 247–251.
Review
For citations:
Koveshnikova E.I., Novik T.S., Napisanova L.A., Chukina S.I., Rudneva O.V. Evaluation of karyopathic effects and general safety of Trichinella spiralis and Echinococcus multilocularis extracts in mice. Russian Journal of Parasitology. 2020;14(4):90-98. (In Russ.) https://doi.org/10.31016/1998-8435-2020-14-4-90-98
Views:
320
Email this article 