Мethodical Recommendations for sampling and Preserving of gastrointestinal Nematodes of Ruminants
https://doi.org/10.31016/1998-8435-2020-14-2-120-124
Abstract
The purpose of the research is improving the accuracy of studies of nematodes parasitizing in the digestive tract of ruminants.
Materials and methods. The literature data and own research results concerning sampling and preserving of nematodes parasitizing various parts of gastrointestinal tract of domestic and wild ruminants have been analyzed. The possibility of using the samples of nematodes, both in morphological and molecular studies, has been taken into account.
Results and discussion. The methods for sampling and preserving of gastrointestinal nematodes of ruminants are described in detail. The features of sampling nematodes parasitizing in various parts of the digestive tract are considered. The ways for minimizing of losses of nematode samples during helminthological necropsy are proposed. Using of 96% ethanol as a preserving liquid is recommended and allows the DNA study of samples.
About the Author
D. N. Kuznetsov
All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants – a branch of Federal State Budgetary Institution of Science "Federal Scientific Center – All-Russian Scientific Research Institute of Experimental Veterinary Medicine named after K. I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences"
Russian Federation
28, B. Cheremushkinskaya st., Moscow, Russia, 117218
Russian Federation
28, B. Cheremushkinskaya st., Moscow, Russia, 117218
References
1. Akbaev M. Sh., Vodyanov A. A., Kosminkov N. E., Yatusevitch A. I., Pashkin P. I., Vasylevitch F. I. Parasitology and invasive diseases of animals. Moscow, 1998; 744. (In Russ.)
2. Govorka Ya., Maklakova L. P., Mitukh Ya., Pelgunov A. N., Rykovskyi A. S., Semenova M. K., Sonin M. D., Erkhardova-Kotrla B., Yurashek V. Helminths of wild ungulates in Eastern Europe. Moscow, 1988; 208 (In Russ.)
3. Ivashkin V. M., Kontrimavicius V. N., Nazarova N. S. Methods of collection and study of helminthes of terrestrial mammals. Moscow, 1971; 124. (In Russ.)
4. Kotelnikov G. A. Helminthological studies of animals and the environment. Moscow, 1984; 208. (In Russ.)
5. Kuznetsov D. N., Danzan G., Batchimeg M., Punsalpaamuu G. Ruminant nematodes in Mongolia are causative agents of helminthozoonoses. Meditsinskaia Parazitologiia i Parazitarnye Bolezni = Medical Parasitology and Parasitic Diseases. 2010; 3: 38–39. (In Russ.)
6. Pryadko E. I. Helminths of deer. Alma-Ata, 1976; 224. (In Russ.)
7. Skrjabin K. I. The method of complete helminthological dissections of vertebrates, including humans. Moscow, 1928; 45. (In Russ.)
8. Skrjabin K. I., Shikhobalova N. P., Shults R. S. Essentials of nematodology III. Trichostrongylids of animals and man. Moscow, 1954; 684. (In Russ.)
9. Skrjabin K. I., Petrov A. M. Essentials of veterinary nematodology. Moscow, 1964; 527. (In Russ.)
10. Hodda M. Phylum Nematoda. Zootaxa. 2007; 1668. 265–293.
11. Irvine R. J., Corbishley H., Pilkington J. G., Albon S. D. Low-level parasitic worm burdens may reduce body condition in free-ranging red deer (Cervus elaphus). Parasitology. 2006; 133. 465–475.
12. Mizani A., Gill P., Daryani A., Sarvi S., Amouei A., Katrimi A. B., Soleymani E., Mirshafiee S., Gholami S., Hosseini S. A., Gholami S., Rahimi M. T., Hashemi-Soteh M. B., Sharif M. A multiplex restriction enzyme-PCR for unequivocal identification and differentiation of Trichostrongylus species in human samples. Acta tropica. 2017; 173. 180–184.
13. Olsen O. W. Animal Parasites. New York: Dover Publications, 1974. 497–502.
Review
For citations:
Kuznetsov D.N. Мethodical Recommendations for sampling and Preserving of gastrointestinal Nematodes of Ruminants. Russian Journal of Parasitology. 2020;14(2):120-124. (In Russ.) https://doi.org/10.31016/1998-8435-2020-14-2-120-124
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