Determination Role of Trichinella larvae at Early Stages of Development in Spread of Trichinellosis

The purpose of the research is the determination of resistance to low temperatures of invasioned nonencapsulated Trichinella spiralis and T. nativа Trichinella larvae in muscular tissue of animals as well as detection of opportunity to develop or keeping these larvae in muscular tissue under different positive temperature conditions. Materials and methods. The material for study and comparison were samples of muscular tissue of white rats experimentally infected by T. spiralis and T. nativа. In total 20 outbred white rats with body weight of 100-150 g were infected in a dose of 10 l/g. Animals were slaughtered in 15th, 16th, 17th, 24th and 30th day after infection. For comparative diagnostics and confidence of experiments only forced meat from hind legs of rats with body weight of 50 g per 1 l of simulated gastric fluid have been taken for peptolysis. The quantity of separated larvae and their morphology have been taking into account during microscopic examination after completion of operation period. Microscope ZEISS Primo Star. was used for microphotographing. Photomicrography of Trichinella separated after digestion on early terms (16-24 days) were made for detailing changes in the morphology of the evaluative larvae. Results and discussion. It hasbeen established that Trichinella larvae of this spice at theageof 17-18 days are nonsustained to low-temperature exploration and died within 24 hours at a temperature -7°С. Infective larvae nonencapsulated or with ill-defined capsule at the age of 24 and 30 days are nonsustained to low-temperature exploration and mainly died at -7-15°С within 24 hours. Nonencapsulated T. spiralis и T. nativа larvae aged 15, 16, and 17 days at positive temperature do not go on morphologically in muscular tissue of murdered animal but they can keep viability, and probably invasiveness in the process of decomposition during some period of time needed to rotation of host and as a result they can be the source of invasion.

трихинеллез, личинки трихинелл, T. spiralis, T. nativа, пептолиз, трихинеллоскопия, trichinellosis, Trichinella larvae, T. spiralis, T. nativа, peptolisis, trichinelloscopy

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