Evaluation of embryotoxic and teratogenic properties triclabendazole suspension

Abstract

Objective of research: To investigate emryotoxic and teratogenic properties of anthelmintic «Triclabendazole suspension of 5%» on white outbred rats. Materials and methods: Experiments were conducted on 72 pregnant albino rats weighing 235-260 g that were divided into six groups: three experimental (by 16 pregnant females each) and three control groups (8 pregnant females each). The rats from experimental groups were injected by suspension into the stomach at a dose of 36 mg/kg (3-fold therapeutic dose for cattle) in critical periods of embryogenesis as follows: 1 to 7 days of pregnancy (I group); 8 to 14 (II group) and from 1 to 19 days of pregnancy (III group). Control animals were injected with a physiological solution in an appropriate volume. Embryotoxic effect of the drug was evaluated by pre-implantation death of zygotes, and post-implantation death of embryos and total embryonic mortality. Teratogenic effect was estimated on organogenesis disorders in utero and changes in bone system of the fetus. Results and discussion: It was found that the general status and behavior of rats receiving the anthelmintic drug “Triclabendazole suspension 5%” at the dose of 36 mg a.i./kg remained without changes in all periods of pregnancy. The animals consumed food and water with pleasure, responded adequately to external stimuli. “Triclabendazole suspension 5%” at the dose of 36 mg a.i./kg as an intragastric injection applied during critical periods of pregnancy does not induce pre-implantation, post-implantation and total embryonic mortality, and does not cause organogenesis disorders in utero and changes in bone system of the fetus.