Veterinary-sanitary expertise of fishes infected by ligula

Objective of research: Comparative studies of qualitative indexes of meat from healthy fish and fish infected with ligulosis for its further sanitary assessment. Materials and methods: As research material served the breams infected with Ligula and free from them (by 30 ind.) caught by amateur fishermen in reservoirs of Moscow region. Fish invasion was studied by K.I.Skryabin method of incomplete helminthological dissection; age of fish was detected on scale; after that weighing was carried out. Microbiological examination of meat from sick and healthy fishes was conducted according to «The instruction for sanitary and microbiological control of food products from fish and sea invertebrates» (Sazonova A. S., etc., 1991). Determination of free and bound amino acids in a water extract of muscular tissue of fishes was carried out by method of a capillary electrophoresis. Toxic elements in fishes were detected by methods of atomic-emission and absorbing spectrometry. Results and discussion: Growth retardation of breams infected with ligulosis rises with age (intensity of infection 1-2 ind. per head); that is displayed in their weight reduction. All unreplaceable amino acids were found in proteins of muscular tissue of fishes. However, fishes infected with ligulosis had lower levels of amino acids that indicates a decrease in nutrition value of a food product. Microbiological research of fish meat showed that MAFAM (number of mesophilic aerobic and facultative anaerobic microorganisms) was higher in muscular tissue of breams infected with ligulosis (10х10⁵), and in meat of healthy fish - 5,5х10³ that did not exceed the maximum allowable concentration values. Bacteria of colibacillus group, staphylococcus, salmonellas and L.monocytogenes in were not determined in all fish samples. In muscle tissue samples isolated from all studied fishes, the levels of lead, cadmium and arsenic did not exceed the maximum allowable concentrations. Mercury wasn’t ever revealed in none of studied samples. The results of our research confirmed the safety of bream meat infected with ligulosis for the consumer.

лещ, лигулез, ремнецы, ветеринарно-санитарная экспертиза, Ligula intestinalis, bream, ligulosis, Ligulidae, veterinary and sanitary examination, Ligula intestinalis

1. Авраменко И.М., Творовский В.С. Экологические проблемы Печенежского водохранилища // Рыбное хозяйство Украины: Тр. Керченского морского техн. ин-та. Керчь, 2000. - С. 56.

2. Васенко О.Г Экологические основы водоохранной деятельности в теплоэнергетике //Х.: Укрнииэп, 2000. - 243 с.

3. Давыдов О.Н., Куровская Л.Я., Базеев РЕ. Лигулез рыб Киевского водохранилища // XII Конф. Укр. наук. тов-ва паразитологов, 10-12 июня 2002 г., Севастополь. К., 2002. - С.32.

4. Ихтиопатология: учебно-методическое пособие / Под ред. В.С. Прудникова, А.В. Мясоедова, B.А. Герасимчик. Витебск, 2001. - 90 с.

5. Канаев А.К. Словарь-справочник ихтиопатолога. - М.: Росагропромиздат, 1988. - 304 с.

6. Котельников ГА. Гельминтологические исследования животных и окружающей среды: Справочник. М.: Колос, 1984. - 208 с.

7. Определитель паразитов пресноводных рыб фауны СССР. Л.: Наука, 1985. Т. 2: Паразитические многоклеточные (Первая часть). - 425 с.

8. Определитель паразитов пресноводных рыб фауны СССР. Л.: Наука, 1987. Т. 3: Паразитические многоклеточные (Вторая часть). 583 с.

9. СанПиН 2.3.2.1078-01 Гигиенические требования безопасности и пищевой ценности пищевых продуктов, Минздрав России, 2002, с. 127

10. СанПиН 3.2.1333-03 Профилактика паразитарных болезней на территории Российской Федерации, Минздрав России, 2003 г., 13 с.

11. Тайгузин Р.Ш., Евграфова З.С., Кучапина Л.А. Ветеринарно-санитарная экспертиза пресноводной рыбы в норме и при лигулезе // Ж. Известия Оренбургского государственного аграрного университета. - 2015. - № 3 (53). - C. 208-209.