Changes of values obtained in Single Cell Gel Electrophoresis of host somatic cells at experimental opisthorchosis
Abstract
On applying the method of alkaline Single Cell Gel Electrophoresis (SCGE) it has been established that metabolites of cat liver fluke maritas have a genotoxic influence on somatic cells of golden hamsters. Genotoxic influence is observed in blood cells of animals on the 7, 14, 21, 28, 35, 60, 90, 120 and 150-th days of inva-sion with the maximum 8,2 times expression on the 14-th day of invasion. The in-dicator «the comets tail moment» in bone marrow cells was 1,9–6,5 times higher than the control values with the maximum expression on the 21-st day of invasion. Maximum genotoxic 7,1 times effect in the liver was observed on the 14-th day of invasion. The level of apoptotic cells in blood, bone marrow and liver of animals in experimental opistorchosis increases due to cytotoxic effect of invasion. Cytotoxic effect of metabolites of cat liver fluke maritas is observed in blood on the 7, 14, 21, 28, 35, 60, 90, 120 and 150-th days with the maximum 8,3 times expression of these changes on the 21-st day. In bone marrow the maximum 10,5 times degree of cells apoptosis was observed on the 14 and 28-th days of invasion. The liver cells apoptosis in infected animals 2,3-6,2 times exceeded the control values with the maximum expression of these changes on 28-th day of observation.
About the Authors
D. K. Kuzhel
Vitebsk State Medical University
Russian Federation
postgraduate
Russian Federation
postgraduate
V. J. Bekish
Vitebsk State Medical University
Russian Federation
doctor of medical sciences
Russian Federation
doctor of medical sciences
V. V. Zorina
Vitebsk State Medical University
Russian Federation
PhD in biological sciences
Russian Federation
PhD in biological sciences
References
1. Durnev A.D. i dr. Primenenie metoda schelochnogo gel-elektroforeza izoli-rovannyh kletok dly ocenki genotoksicheskih svoystv prirodnyh i sinteticheskich soedineniy. Metod. rekomendacii. – M., 2006. – 27 s.
2. Ilinskih E.N. i dr. Vliynie associacii trematod Opisthorchis felineus i poten-cialno onkogennogo virusa Epschteyna Barr na uroven citogeneticheskih pora-zcheniy T-limfocitov cheloveka // Sb. dokl. «Dostizheniy i perspektivi razvitiy sov-remennoy parazitologii». – Vitebsk: VGMU, 2006. – S. 71–73.
3. Ilinskih E.N. i dr. Ekogenetika opistorchoza i persistenciy virusa Ep-schteyna-Barr. – Tomsk: Izd. Sib. med. un-ta, 2000. – 268 s.
4. Ilinskih N.N. Populycionnie issledovaniy citogeneticheskoy patalogii v ochagah opistorhoza v usloviyh Ob-Irtishskogo basseyna // Sb. dokl. «Komplex. gigien. issled. v prakt. zdravoohr». – Novokuzneck, 1981. – S. 481–484.
5. Ilinskih N.N. Problema opistorchoza na severe Tymenskoy oblasti v svyzi s ego vliyniem na geneticheskie struktury organizma // Sb. dokl. «Osobennosti patologii u korennogo i prishlogo naseleniy v usloviyh Kraynego Severa». – Kras-noyrsk, 1981. – T. 2. – S. 198.
6. Kuzhel D.K. Experimentalnay model opistorhoza na zolotistyh homykah // Mater. X Mezhdunar. nauch.-prakt. konf. «Agrarnoe proizvodstvo i ochrana priro-dy». – Vitebsk: VGAVM, 2011. – S. 96–97.
7. Palcev A.I. i dr. Patomorfoz opistorhoza // Med. parazitol. i parazit. bol. – 1994. – № 1. – S. 29–33.
8. Pashinskay E.S. i dr. Primenenie schelochnogo gel-elektroforeza izoliro-vannyh kletok v embrionalnyh tkanyh myshey // Mater. 62 nauch. sessii UO «VGMU» «Dostizheniy fundamentalnoy klinicheskoy mediciny i farmacii». – Vi-tebsk, 2007. – S. 163–166.
9. Solov’eva A.V. Hronicheskiy opistorhoz i beremennost. Rukovodstvo dly vrachey. – M.: GEOTAR-Media, 2007. – 130 s.
10. Ykubovskiy M.V., Skurat E.K. Opistorhoz: opasnost zarazheniy i profil-aktika // Vet. med. Belarusi. – 2008. – № 1–2. – S. 6–11.
11. Chen L. et al. Skin-stage schistosomula of Schistosoma mansoni produce an apoptosis-inducing factor that can cause apoptosis of T cells // J. Biol. Chem. – 2002. – V. 277, № 37. – P. 34329–34335.
12. Florent M. et al. Detection by the comet assay of apoptosis induced in lymphoid cell lines after growth factor deprivation // Cell Biol. Toxicol. – 1999. – V. 15. – P. 185–192.
13. Lundy S.K., Lerman S.P., Boros D.L. Soluble egg antigen-stimulated T helper lymphocyte apoptosis and evidence for cell death mediated by FasL ± T and B cells during murine Schistosoma mansoni infection // Infection and Immunity. – 2001. –V. 69, № 1. – P. 271–280.
14. Motorna O.O. et al. Analysis of lacI mutations in Big Blue® transgenic mice subjected to parasite-induced inflammation // Mutat. Res. Fund. and Mol. Mech. of Mutagen. – 2001. – V. 484. – P. 69–76.
Review
For citations:
Kuzhel D.K., Bekish V.J., Zorina V.V. Changes of values obtained in Single Cell Gel Electrophoresis of host somatic cells at experimental opisthorchosis. Russian Journal of Parasitology. 2014;(3):52-58. (In Russ.)
Views:
180
Email this article 