Evaluation of anthelmintic efficacy of solid dispersion of albendazole in experimental fasciolosis and spontaneous dicrocoeliosis of sheep

The purpose of the research is to study the anthelmintic efficacy of solid dispersion of albendazole in experimental fasciolosis and dicrocoeliosis of sheep.

Materials and methods. A solid dispersion of albendazole was obtained by mechanochemical processing of albendazole and polyvinylpyrrolidone (1 : 9) in a roller mill LE-101 at an energy intensity level of 1 g for 4 hours at a drum rotation speed of 60–70 rpm. The activity of albendazole solid dispersion against immature and mature Fasciola hepatica was studied on 30 white rats experimentally infected with fasciolae at a dose of 20 adolescaria per animal. The drugs were administered on 4 and 12 weeks after infection. Animals that did not receive the drugs served as controls. The efficacy of the drugs was assessed on 5 and 13 weeks after infection during helminthological necropsy of animal livers. The efficacy of solid dispersion of albendazole was studied on 40 sheep naturally infected with dicrocoeliosis at doses of 5 and 10 mg/kg of active substance (AS) in comparison with the control and the basic drug – albendazole substance. The efficacy of the drugs was studied based on the results of coproovoscopy before and 20 days after treatment.

Results and discussion. . It was found that the solid dispersion of albendazole has 92.3% efficacy against mature fasciolae, against immature ones its efficacy was 49.26%. The basic drug at a dose of 10 mg/kg showed insufficient activity against adults – 53.8% and immature fasciolae – 16.4%. The significant increase in the efficacy of solid dispersion of albendazole was recorded against dicrocoeliosis of sheep. The studied drug showed 79.5% effect at a dose of 5 mg/kg of AS, i.e. practically the same efficiency as the substance of albendazole at a dose of 10 mg/kg. The solid dispersion of albendazole showed 97.0% efficacy at a dose of 10.0 mg/kg of AS.

1. Arkhipov I. A. Efficiency of valbazen at fasciolosis, dictyocaulosis, monieziosis and strongylosis of the gastrointestinal tract of sheep. Bulletin of the All-Union Institute of Helminthology = Bulletin of the All-Union Institute of Helminthology. 1996; 56: 8–11. (In Russ.)

2. Arkhipov I. A. Efficiency of microencapsulated albendazole plus at strongylosis of the gastrointestinal tract of sheep and fasciolosis of cows. Materials of the reports of the scientific conference of the All-Russian Society of Helminthologists of the Russian Academy of Sciences "Theory and Practice of Combating Parasitic Diseases" = Proceedings of the scientific conference of the All-Russian Society of Helminthologists of the Russian Academy of Sciences “Theory and Practice of Combating Parasitic Diseases”. Moscow, 1999; 18–20. (In Russ.)

3. Arkhipov I. A. Anthelmintics: pharmacology and application. M.: Publishing house of the Russian Academy of Agricultural Sciences, 2009; 409. (In Russ.)

4. Astafiev B. A., Yarotsky L. S., Lebedeva M. N. Experimental models of parasitoses in biology and medicine. M.: Nauka, 1989; 278. (In Russ.)

5. Kotelnikov G. A. Helminthological studies of animals and the environment. M.: Kolos, 1984; 208. (In Russ.)

6. Orobets V. A., Sidorkin V. A. Efficiency of alvet suspension at dicrocoeliosis of sheep. Materials of the reports of the scientific conference of the All-Russian Society of Helminthologists of the Russian Academy of Sciences "Theory and Practice of Combating Parasitic Diseases" = Proceedings of the scientific conference of the All-Russian Society of Helminthologists of the Russian Academy of Sciences "Theory and Practice of Combating Parasitic Diseases". M., 2004; 5: 295-297. (In Russ.)

7. Sidorkin V. A. Handbook of diagnostics and therapy of helminthosis of animals and birds. Moscow: Aquarium, 2001; 44. (In Russ.)

8. Shekhovtsov V. S., Lutsenko L. I., Mishareva T. Ya. Efficiency of albendazole in helminthosis of sheep. Veterinary science. 1990; 5: 69–71. (In Russ.)

9. Bradley R. E, Randell W. F., Armstrong D. A. Anthelmintic efficacy of albendazole in calves with naturally acquired Fasciola hepatica infections. American Journal of Veterinary Research. 1981; 42 (6): 1062-1064.

10. Chistyachenko Y. S., Meteleva E. S., Pakharukova M. Y., Katokhin A. V., Khvostov M. V., Varlamova A. I., Glamazdin I. I., Khalikov S. S., Polyakov N. E., Arkhipov I. A., Tolstikova T. G., Mordvinov V. A., Dushkin A. V., Lyakhov N. Z. A Physicochemical and pharmacological study of the newly synthesized complex of albendazole and the polysaccharide arabinogalactan from Larch Wood. Current Drug Delivery. 2015; 12 (5): 477-90. https://doi.org/10.2174/1567201812666150518094739

11. Cook G. C. Use of benzimidazole chemotherapy in human helminthiases: Indications and efficacy. Parasitology Today. 1990; 6: 133–136.

12. Corba J. Krupicer I., Legeni I., Juris P., Vesely L. Effect of a controlled release albendazole capsule on parasitism and productivity of sheep. Veterinary Parasitology. 1991; 40 (3/4): 273–279.

13. Dzakula N., Rapic D., Zukovic M., Blagovic S., Tasdic M., Rendic Z., Stojcevic D. Efficacy of albendazole (Monil) and closantel hydroxide (Fascoverm) against Dicrocoelium dendriticum in sheep. Veterinarski Arhiv. 1984; 54 (2): 105–115.

14. Geurden T., Hoste H., Jacquiet P., Traversa D., Sotiraki S., Frangipane di Regalbono A., Tzanidakis N., Kostopoulou D., Gaillac C., Privat S., Giangaspero A., Zanardello C., Noé L., Vanimisetti B., Bartram D. Anthelmintic resistance and multidrug resistance in sheep gastro-intestinal nematodes in France, Greece and Italy. Veterinary Parasitology. 2014; 17; 201 (1-2): 59-66. https://doi.org/10.1016/j.vetpar.2014.01.016.

15. Gyurik R. J., Theodorides V. J. Methyl 5-propylthio2- benzimidazolcarbamate. US patent, ed.: SmithKline Corporation. 1975.

16. Herlich H. Anthelmintic efficacy of albendazole in comparison of critical and controlled test. American Journal of Veterinary Research. 1977; 38 (8): 1247–1248.

17. Himonas C., Liakos V. Efficacy of albendazole against Dicrocoelium dendriticum in sheep. Veterinary Record. 1980; 107 (12): 288–289. https://doi.org/10.1136/vr.107.12.288

18. Knight R. A., Colglazier M. L. Albendazole as a fasciolicide in experimentally infected sheep. American Journal of Veterinary Research. 1977. 38 (6): 807–808.

19. Lindemberg M., Kopp S., Dressman J. B. Classification of orally administered drugs on the World Health Organization Model list of Essential Medicines according to the biopharmaceutics classification system. European Journal of Pharmaceutics and Biopharmaceutics. 2004; 58 (2): 265–278. https://doi.org/10.1016/j.ejpb.2004.03.001

20. Marriner S. Anthelmintic drugs. Veterinary Record. 1986; 118 (7): 181–184. https://doi.org/10.1136/vr.118.7.181

21. O’Neil M. J. The Merck Index. 40th edition. Whitehouse Station, NJ: Merch Research Laboratoires. 2006; 39.

22. Petermann J., Grisez C., Lavigne S., Jacquiet P. Lack of Efficacy of Albendazole against Dicrocoelium dendriticum infection in a sheep farm in France. Animals (Basel). 2024; 14 (13): 1992. https://doi.org/10.3390/ani14131992

23. Pranzo M. B., Cruickshank D., Coruzzi M., Caira M. R., Bettini R. Enantiotropically related albendazole polymorphs. Journal of Pharmaceutical Science. 2010; 99 (9): 3731-3742. https://doi.org/10.1002/jps.22072

24. Tharaldsen J. A., Wethe J. A. A field trial with albendazole against Dicrocoelium lanceatum in sheep. Nordisk Veterinaermedicin. 1980; 32 (7-8): 308-312.

25. Theodorides V. J., Gyurik R. J., Kingsbury W. D., Parish R. C. Anthelmintic activity of albendazole against liver flukes, tapeworms, lung and gastrointestinal roundworms. Experientia. 1976; 32 (6): 702-703. https://doi.org/10.1007/BF01919842

26. Theodorides V. J., Bell R. R., Chemey J. M., Ciordia H. Spectrum activity of albendazole. Proceedings of the 8th International Conference of the World Association for the Advancement of Veterinary parasitology. Sydney. 1977; 3: 935.

27. Theodorides V. J., Freeman J. F. Efficacy of albendazole against Fasciola hepatica in cattle. Veterinary Record. 1980; 106 (4): 78-79. https://doi.org/10.1136/vr.106.4.78

28. Theodorides V. J., Freeman J. F., Georgi J. R. Anthelmintic activity of albendazol against Dicrocoelium dendriticum in sheep. Veterinary medicine small animal clinician. 1982; 77: 569–570.

29. Torrado S., Torrado S., Torrado J. J., Caerdoniga R. Preparation, dissolution and characterization of albendazole solid dispersions. International Journal of Pharmaceutics. 1996; 140: 247–250. https://doi.org/10.1016/0378-5173(96)04586-3

30. World Association for the Advancement of Veterinary Parasitology (W. A. A. V. P.) second edition of guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine, ovine, caprine). Journal of Veterinary Parasitology. 1995; 58. 181-213.