Biochemical blood parameters in chronic Opisthorchis felineus infection in rabbits in the experiment

The purpose of the research is to analyze biochemical blood parameters in the chronic opisthorchosis stage in long-term infection in a compensation status by the example of the rabbit.

Materials and methods. The experiment compared the control and experimental groups of sexually mature rabbits (Oryctolagus cuniculus domesticus), 10 animals each. The rabbits were infected with 50 metacercariae of Opisthorchis felineus S. Rivolta, 1884. Five years after infection, a biochemical analyzer determined glucose, creatinine, urea, blood urea nitrogen to creatinine ratio, total calcium and phosphorus, total protein, albumin, globulin, albumin to globulin ratio, alanine aminotransferase, alkaline phosphatase, gamma-glutamine transferase, bilirubin, and cholesterol in the animals' blood plasma.

Results and discussion. The blood plasma in the experimental rabbits was found to have statistically significantly increased urea, the urea nitrogen to creatinine ratio, albumins, and the albumins to globulins ratio versus the control group. Glucose, creatinine, phosphorus, calcium, alkaline phosphatase, and cholesterol were higher in the experimental group. The data were not statistically significant. On the contrary, the amount of globulins, alanine aminotransferase, total protein and total bilirubin was lower versus the control group. The evaluation of bilirubin fractions between the groups found a lower indirect fraction versus the control. The data analysis results developed a diagnostic panel where probable involvement in the pathological process was assessed for the host's organs and systems using the example of O. felineus infection based on the values of biochemical blood parameters. The biochemical blood parameters in the chronic stage of opisthorchosis were normal or deviated slightly in a comprehensive snapshot analysis.

1. Bakshtanovskaya I. V., Stepanova K. B., Kalgi- na G. A., Stepanova T. F. Interrelations of biochemical and immunological parameters in patients with chronic opisthorchosis. Meditsinskaya parazitologiya i parazitarnyye bolezni = Medical parasitology and parasitic diseases. 2018; 2: 13-19. (In Russ.)

2. Bekish O.-Ya. L., Semenov V. M., Bekish L. E., Bekish V. Ya. The impact of helminths on vitamin metabolism in their hosts. Vestnik Vitebskogo gosudarstvennogo meditsinskogo universiteta = Bulletin of the Vitebsk State Medical University. 2007; 6 (3): 86-92. (In Russ.)

3. Beer S. A. Biology of the causative agent of opisthorchosis. M.: Scientific Publishing Association of the Mass Cultural Committee, 2005; 336. (In Russ.)

4. Bogdanov A. O., Prokudina D. V., Baikov A. N., Saltykova I. V. Molecular mechanisms that mediate the development of cholangiocarcinoma in chronic liver fluke infection. Sibirskiy onkologicheskiy zhurnal = Siberian Journal of Oncology. 2015; 6: 83 90. (In Russ.)

5. Borzunov V. M., Verevshchikov V. K., Kuzne- tsov P. L., Udilov V. S., Ruslyakov D. V., Solda- tov D. A., Belousov V. V. Helminth infections and protozoan diseases in humans. Ekaterinburg: Ural State Medical University, 2013; 148. (In Russ.)

6. Bychkov V. G. Opisthorchosis and liver cancer in hyperendemic focus population. Novosibirsk: Science, 1992; 175. (In Russ.)

7. Vishnevskaya T. Ya., Abramova L. L. Hematological blood factors in rabbits under stress and in its immune correction. Aktual'nyye problemy intensivnogo razvitiya zhivotnovodstva = Current issues of intensive animal breeding development. 2012; 15 (2): 203-208. (In Russ.)

8. Voitenko N. G., Makarova M. N., Kovaleva M. A. Variability of biochemical blood parameters and setting reference intervals in preclinical studies. Message 2: rabbits. Laboratornyye zhivotnyye dlya nauchnykh issledovaniy = Laboratory animals for scientific research. 2020; 2: 3-10. (In Russ.) https://doi.org/10.29296/2618723X-2020-02-01

9. Ivanskikh V. I. Studying the surface of Opisthorchis felineus cysts and excysted metacercariae by scanning electron microscopy. Parazitologija = Parasitology. 1993; 27 (1): 54-58. (In Russ.)

10. Ishmanov M. Yu., Sertakova A. V., Soloviev A. M., Fedyashina N. A., Shcherbakova E. V. 250 Health Indicators. Directory. Litres, 2013; 718. (In Russ.)

11. Kosarenko E. S., Zuevskaya T. V., Orlov M. D., Eremeev S. I., Romanchenko S. S. Laboratory characteristics of autoimmune liver diseases associated with Opisthorchis infection. Prakticheskaya meditsina = Practical Medicine. 2023; 21 (4): 63-71. (In Russ.)

12. Kuvshinov D. Yu., Sidelnikova A. A. Comparative analysis of blood cell morphology and biochemical characteristics in rabbits with chronic opisthorchosis. Morfologicheskiye vedomosti = Morphological Journal. 2022; 30 (3): 44-49. (In Russ.) https://doi.org/10.20340/mv-mn.2022.30(3).603

13. Maksimova G. A., Pakharukova M. Yu., Kashi- na E. V., Zhukova N. A., Lvova M. N., Khvo- stov M. V., Baev D. S., Katokhin A.V., Tolstikova T. G., Mordvinov V. A. Morphofunctional and biochemical parameters in golden hamsters in the development of opisthorchiasis-associated cholangiocarcinoma. Vavilovskiy zhurnal genetiki i selektsii = Vavilov Journal of Genetics and Breeding. 2015; 19 (4). (In Russ.) https://doi.org/10.18699/VJ15.062

14. Nacheva L. V., Dodonov M. V., Vorobyova E. I. A xenoparasitic barrier in opisthorchiasis (histological and histochemical studies of the vital tripod: liver, pancreas gland, and duodenum). Kemerovo: KemSMA Publishing House, 2009; 137. (In Russ.)

15. Paltsev A. I. Chronic opisthorchosis from the standpoint of a system approach. Clinical picture, diagnostics, pathomorphosis, treatment. Russkiy meditsinskiy zhurnal = Russian Medical Journal. 2005; 2: 96. (In Russ.)

16. Sedov Yu. D. Rabbits. Breeding, Maintenance, Care. Rostov-on-Don: Phoenix, 2016; 173. (In Russ.) 17. Serov V. V., Shekhter A. B. Connective Tissue. M.: Medicine, 1981; 312. (In Russ.)

17. Sidelnikova A. A., Nacheva L. V., Boborykin M. S. Clinical aspects of acute opisthorchiasis in rabbits in the experiment. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2016; 37 (3): 374-379. (In Russ.) https://doi.org/10.12737/21661

18. Sidelnikova A. A., Nacheva L. V., Boborykin M. S. Psychomotor impairments in experimental animals with opisthorchiasis. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2018; 12 (4): 84–89. (In Russ.) https://doi.org/10.31016/1998-8435-2018-12-4-84-89

19. Stepanova T. F., Bakshtanovskaya I. V., Stepano- va K. B., Chirko Yu. V., Arapova O. N. Biochemistry of bile secretion in Opistorchis spp. infection. Fundamental'nyye issledovaniya = Fundamental Research. 2006; 1: 37-38. (In Russ.)

20. Filimonova L. A., Borisenko N. A. Opisthorchosis, clinical manifestations. Sibirskoye meditsinskoye obozreniye = Siberian Medical Review. 2006; 39 (2): 8-12. (In Russ.)

21. Frolova O. V., Startseva O. N. Comparative characteristics of biochemical blood parameters in patients with acute and chronic opisthorchosis. Fundamental'nyye issledovaniya = Fundamental Research. 2004; 3: 121-122. (In Russ.)

22. Tsukanov V. V., Tonkikh Yu. L., Gilyuk A. V., Vasyutin A. V., Kolenchukova O. A., Rzhaviche- va O. S., Borisov A. G., Savchenko A. A., Maslennikova N. A. Opisthorchosis diagnostics, clinical picture, and treatment. Doktor.Ru = Doctor. Ru. 2019; 8 (163): 49-53. (In Russ.)

23. Ewringmann A. Leitsymptome beim Kaninchen: Diagnostischer Leitfaden und Therapie, Enke; Auflage: 2., September 2010. 25. Dieker A., Steinkamp J. Unzez Zwergkaninchen. Studgard: Franckh-Kosmos Verlage. Ymbn. 1994; 72.

24. Von Dietrich Altman. Zwerg-Kaninchen. Studgard: Ulmer,1996; 48.

25. Hein J., Hartmann K. Aus der I. Medizinischen Tierklinik (Vorstand: Prof. Dr. K. Hartmann) der Ludwig-Maximilians-Universität München. Tierärztliche Praxis Kleintiere, Heft. № 5, 2003.

26. Turley S. D., West C. E., Horton B. J. The role of ascorbic acid in the regulation of cholesterol metabolism and in the pathogenesis of artherosclerosis. Atherosclerosis. 1976; 24 (1-2): 1-18. https://doi.org/10.1016/0021-9150(76)90060-5