Deacarization-associated sanogenetic status of the organism of laying hens with dermanyssosis

The purpose of the research is to describe changes in the sanogenetic status of the organism of Hysex White laying hens with dermanyssosis as associated with deacarization with a new D-cyphenothrin, piperonyl butoxide and pyriproxyfenbased insectoacaricide.

Materials and methods. The research was conducted in the industrial sector; an average tick infestation with Dermanyssus gallinae was found in a poultry building where 20-month-old Hysex White hens were kept. Then, five laying hens with dermanyssosis from this building were transported to the Podolsk Experimental Production Base of the VNIIP. Blood samples were collected from the birds before treatment and at 15 and 30 days after double treatment (7-day interval) with 0.005% aqueous emulsion of a D-cyphenothrin, piperonyl butoxide and pyriproxyfen-based product. The building and the birds were treated by fine-droplet spraying at a rate of 25–50 mL/m². Some blood parameters were studied over time.

Results and discussion. The treatments were associated with an improved overall health status of the hens, increased synthetic activity of the thyroid gland, stabilized oxidative stress indicators, normalized morpho-physiological blood parameters, and intensified carbohydrate and energy balance, and lipid and protein metabolism as compared to their state in the industrial sector before the treatments. The blood changes in the hens after the treatment reflected the activation of a complex of adaptive and restorative mechanisms in their organism. The hens had no feathers before the treatments, but within one month after the treatments, three out of five birds had almost completely restored their plumage, while the rest still had bold areas on the skin. Thus, the new insectoacaricide is effective against D. gallinae and has no negative effect on laying hens, while sanogenetic mechanisms for homeostasis stabilization are activated in their organism.

1. Arisov M. V., Indyuhova E. N., Arisova G. B., Poselov D. S., Stepanov A. A., Poselova E. V. Acute oral and dermal toxicity parameters of a drug based on D-cyphenothrin, pyriproxyfen and piperonyl butoxide in laboratory animals. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2024; 18 (4):410–418. (In Russ.) https://doi.org/10.31016/1998-8435-2024-18-4-410-418

2. Baranovsky A. Yu., Kruglova N. A., Grigorieva E. Yu. A variant of functional nutrition for patients with non-alcoholic fatty liver disease. Eksperimental'naya i klinicheskaya gastroenterologiya = Experimental and Clinical Gastroenterology. 2024; 10: 189-196. (In Russ.) https://doi.org/10.31146/1682-8658 ecg-218-10-189-196

3. Buslovskaya L. K., Kovtunenko A. Yu., Belyaeva E. Yu. Adaptation of chickens to industrial maintenance factors. Regional'nye geosistemy = Regional geosystems. 2010; 13(21(92)): 96-102. (In Russ.)

4. Gudin V. A., Lysov V. F., Maximov V. I. Physiology and ethology of poultry. SPb.: Lan, 2010; 336. (In Russ.)

5. Gusakova E. A., Gorodetskaya I. V. Severity comparison of the stress-protective effect of thyroxine and hydrocortisone. «BGMU v avangarde medicinskoj nauki i praktiki»: sbornik nauchnyh trudov = "BSMU at the Forefront of Medical Science and Practice": сollection of scientific papers. 2019; 9: 166-171. (In Russ.)

6. Delis I. V., Ryzhkova G. F. Activity of some enzymes in poultry when B-Traxim Se and tocopherol are included in the diet. Vestnik Kurskoj gosudarstvennoj sel'skohozyajstvennoj akademii = Bulletin of the Kursk State Agricultural Academy. 2015; 2: 59-61. (In Russ.)

7. Zaitseva N. V., Lanin D. V., Chereshnev V. A. Immune and neuroendocrine regulation when exposed to chemical factors of various origins. Perm: Publishing House of the Perm National Research Polytechnic University, 2016; 236. (In Russ.)

8. Indyuhova E. N., Arisov M. V., Azarnova T. O., Maximov V. I. Sanogenetic basis for correction of physiological and biochemical status in hens with dermanyssosis. M.: Nauka, 2024; 242 (In Russ.). https://doi.org/10.31016/978-5-6050437-5-1-2024-242

9. Karmoliev R. Kh. Modern biochemical research methods in veterinary science and zootechnics. M.: Kolos, 1971; 288. (In Russ.)

10. Kondratenko E. I., Teply D. L., Maslov A. K. Experimental evaluation of the functional status of the thyroid gland in white rats upon administration of antioxidants, triiodothyronine, and their combinations. Uchenye zapiski = Proceedings. 1996; 2: 47-55. (In Russ.)

11. Kondrakhin I. P. Methods of veterinary clinical laboratory diagnostics. M.: KolosS, 2004; 520. (In Russ.)

12. Lygdenov D. V., Sordonova E. V., Zhamsarano- va S. D. The effect of iodine and zinc organic forms on the ratio of pro-oxidant and antioxidant systems in the organism with iodine deficiency. Izvestiya vu zov. Prikladnaya himiya i biotekhnologiya = News from universities. Applied chemistry and biotechnol ogy. 2017; 7(4(23)): 36-43. (In Russ.)

13. Patent No. 2151502 Russian Federation, IPC A01K 67/02. Method for predicting stress in laying hens: No. 99105140/13: application dd. 16/03/1999: published 27/06/2000 / V. I. Shcherbatov, L. I. Sidorenko, E. V. Levchenko, T. I. Pakhomova, T. A. Kutovenko, M. N. Jolova; Applicant the Kuban State Agrarian University. 4 p. (In Russ.)

14. Patent No. 2399203 Russian Federation, IPC A01K 67/00, A61D 99/00. Method for assessing the physiological status of the chicken organism: No. 2009108991/13: application dd. 11/03/2009: published 20/09/2010 / V. N. Laskavy, M. L. Malinin; Applicant, State Scientific Institution the Saratov Research Veterinary Station of the Russian Academy of Agricultural Sciences. 7 p. (In Russ.)

15. Rosly I. M., Vodolazhskaya M. G. Rules for reading biochemical analysis. M.: Meditsinskoye informatsionnoye agenstvo, LLC Information Agency), 2020; 112. (In Russ.) (Medical 16. Saifutdinova L.N., Derkho M. A. Biocommunication evaluation of corticosterone and cortisol in the chicken organism in stress response. Uchenye zapiski Kazanskoj gosudarstvennoj akademii veterinarnoj mediciny im. N. E. Baumana = Scientific notes of the Kazan State Academy of Veterinary Medicine named after N. E. Bauman. 2021; 246(2): 187-193. (In Russ.) https://doi.org/10.31588/2413-4201-1883-246-2-187-193

16. Sereda T. I., Derkho M. A. Characteristics of carbohydrate metabolism in the Lohmann white laying hen organism. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta = Bulletin of the Orenburg State Agrarian University. 2011; 3 (31-1): 334-337. (In Russ.)

17. Smirnova T. S., Degtyar Yu. V., Sharaevskaya M. V., Kapitonova M. Yu. Immunohistochemical charac teristics of the thyroid gland in chronic stress. Vest nik Volgogradskogo gosudarstvennogo medicinskogo universiteta = Bulletin of the Volgograd State Medical University. 2008; 4 (28): 51-54. (In Russ.)

18. Tremasova A. M., Beletsky S. O., Ivanov A. A., Kakhaberidze V. V. Sorbents used in rearing young poultry. Ptica i pticeprodukty = Poultry and Chicken Products. 2012; 3 (17); 17-18. (In Russ.)

19. Fomina K. A., Romashko A. A. The effects of gluco corticoids on endocrine regulation. Morfologicheskij al'manah imeni V. G. Koveshnikova = Morphological Almanac named after V.G. Koveshnikov. 2019; 17 (3): 75-77. (In Russ.)

20. Shushkevich N. I. Biochemistry of hormones. Vladimir: Publishing House of the Vladimir State University, 2009; 68. (In Russ.)

21. Arzour-Lakehal N., Siliart B., Benlatreche C. Relationship between plasma free thyroxine levels and some biochemical parameters in two strains of broiler chickens. Global Veterinaria. 2013; 10 (3): 243-249.

22. Gu Y. F., Chen Y. P., Jin R., Wang C., Wen C., Zhou Y. M. Age-related changes in liver metabolism and antioxidant capacity of laying hens. Poultry Science. 2021; 100 (12): 101478.

23. Pavlićević A., Ratajac R., Stojanov I., Pavlovic I. The control program of red poultry mite (Dermanyssus gallinae), today. Archives of Veterinary Medicine/ Arhiv Veterinarske Medicine. 2018; 11 (2): 71-88.

24. Thrall M. A., Weiser G., Allison R. W., Campbell, T. W. (Eds.). Veterinary Hematology, Clinical Chemistry, and Cytology. United Kingdom: John Wiley & Sons, 2022; 1056.

25. Tok S., Şekeroğlu A., Duman M., Tainika B. Effect of age, stocking density, genotype, and cage tier on feather score of layer pure lines. Turkish Journal of Veterinary & Animal Sciences. 2022; 46 (1): 115 123.

26. Tóthová C., Sesztáková E., Bielik B., Nagy O. Changes of total protein and protein fractions in broiler chickens during the fattening period. Veterinary world. 2019; 12 (4): 598-604. https://doi.org/10.14202/vetworld.2019.598-604

27. Wodzicka-Tomaszewska M., Stelmasiak T., Cumming R. B. Stress by immobilization, with food and water deprivation, causes changes in plasma concentration of triiodothyronine, thyroxine and corticosterone in poultry. Australian journal of biological sciences. 1982; 35 (4): 393-402.