Study of susceptibility of siberian pine (Pinus sibirica), scots pine (Pinus sylvestris) and siberian larch (Larix sibirica) seedlings to the pine wilt disease
https://doi.org/10.31016/1998-8435-2025-19-1-125-138
Abstract
The purpose of the research is to investigate the resistance of Pinus sylvestris, P. sibirica and Larix sibirica pine seedlings to the nematode Bursaphelenchus xylophilus to assess the potential risk of death of these conifers from pine wilt disease.
Materials and methods. In the experiment, 8-year-old seedlings of P. sylvestris, P. sibirica and 4–5-year-old seedlings of L. sibirica were used in an amount of 16 seedlings of each species (8 B. xylophilus-infected plants and 8 control plants).
Results and discussion. Pines Pinus sylvestris, P. sibirica and L. sibirica were susceptible to the pine wood nematode B. xylophilus. All plants died by the end of the study, but the most rapid wilting and death of plants was observed in Siberian larch seedlings. More intensive nematode reproduction was recorded in P. sibirica, with seedlings dying later than other plants. The preliminary analysis showed that the death of conifer stands of the studied species from pine wilt disease is possible in the climatic zone of Siberia.
Supporting agencies: The study was supported by State Assignment No. 124030400032-1
About the Authors
O. A. Kulinich
All-Russian Plant Quarantine Center; Center for Parasitology, Institute of Ecology and Evolution A.N. Severtsov Russian Academy of Sciences
Russian Federation
Russian Federation
Kulinich Oleg A. - Doctor of Biological Sciences, Chief Researcher, Forest Quarantine Department, SPIN: 7656-5245, Researcher ID: A-5534-2016, Scopus ID: 11940984400.
Moscow
E. N. Arbuzova
All-Russian Plant Quarantine Center
Russian Federation
Russian Federation
Arbuzova Elena N. - Candidate of Biological Sciences, Senior Researcher, Forest Quarantine Department, SPIN: 3315-4190, Researcher ID: I-7153-2015, Scopus ID: 57222500674.
Moscow
N. I. Kozyreva
All-Russian Plant Quarantine Center
Russian Federation
Russian Federation
Kozyreva Natalya I. - Candidate of Biological Sciences, Researcher, Forest Quarantine Department, SPIN: 6645-9389, Researcher ID: JWP-5077-2024, Scopus ID: 57190217222.
Moscow
A. A. Chalkin
All-Russian Plant Quarantine Center
Russian Federation
Russian Federation
Chalkin Andrey A. - Researcher, Forest Quarantine Department, SPIN: 8575-3984, Researcher ID: AET-9683-2022, Scopus ID: 57220116459.
Moscow
A. G. Shchukovskaya
All-Russian Plant Quarantine Center
Russian Federation
Russian Federation
Shchukovskaya Anastasia G. - Candidate of Biological Sciences, Senior Researcher, Forest Quarantine Department, SPIN: 7346-9724.
Moscow
D. I. Ryaskin
Voronezh branch of the All-Russian Plant Quarantine Center
Russian Federation
Russian Federation
Ryaskin Dmitry I. - Junior Researcher, Scientific and Methodological Department, SPIN code: 5177-3463.
Voronezh
References
1. Kulinich O. A. Parasitic nematodes of conifers and their distribution in the European part of the USSR. Problems of phytohelminthology = Problemy fitogel'mintologii. Moscow, Nauka, 1989; 105–131. (In Russ.)
2. Weather schedule [website]. Weather archive [updated 05 December 2024; cited 05 December 2024]. Available: https://rp5.ru
3. Akiba M., Ishihara M., Sahashi N., Nakamura K., Ohira M., Toda T. Virulence of Bursaphelenchus xylophilus isolated from naturally infested pine forests to five resistant families of Pinus thunbergii. Plant Disease. 2012; 96 (2): 249–252. https://doi.org/10.1094/PDIS-12-10-0910
4. Akiba M., Nakamura K. Susceptibility of adult trees of the endangered species Pinus armandii var. amamiana to pine wilt disease in the field. Journal of Forest Research. 2005; 10: 3–7. https://doi.org/10.1007/s10310-004-0089-8
5. Arbuzova E. N., Kulinich O. A., Chalkin A. A., Kozyreva N. I., Gorbach V. V., Ryss A. Y. Infestation of pine (Pinus sylvestris L.) seedlings with the pinewood nematode Bursaphelenchus xylophilus Steiner and Buhrer (Nickle) through wood sawdust. Annals of Forest Science. 2023; 80 (1): 1-11. https://doi.org/10.1186/s13595-023-01174-y
6. Evans H. F., McNamara D. G., Braasch H., Chadoeuf J., Magnusson C. Pest risk analysis (PRA) for the territories of the European Union (as PRA area) on Bursaphelenchus xylophilus and its vectors in the genus Monochamus. EPPO Bulletin. 1996; 26: 199–249. https://doi.org/10.1111/j.1365-2338.1996.tb00594.x
7. Fonseca L., Cardoso J. M. S., Lopes A., Pestana M., Abreu F., Nunes N., Mota M., Abrantes I. The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island. Helminthologia. 2012; 49: 96–103. https://doi.org/10.2478/s11687-012-0020-3
8. Futai K. Pine wilt disease and the decline of pine forests: a global issue. – Cambridge Scholars Publishing, 2021.
9. Hopf‐Biziks A, Schröder T, Schütz S. Long‐term survival and non‐vector spread of the pinewood nematode, Bursaphelenchus xylophilus, via wood chips. Forest Pathology. 2017; 47 (4): e12340. https://doi.org/10.1111/efp.12340
10. Kobayashi F. The Japanese pine sawyer. Dynamics of forest insect populations: patterns, causes, implications. Boston, M.A.: Springer US, 1988; 431–454.
11. Kulinich O. A., Orlinski P.D. Pathogenicity and possible adaptation of the pinewood nematode, Bursaphelenchus xylophilus, in central European Russia. Protection of world forests from pests: advances in research, IUFRO World Series. Vienna, 2001, 11: 185–193.
12. Linit M. J., Tamura H. Relative susceptibility of four pine species to infection by pinewood nematode. Journal of Nematology. 1987; 19 (1): 44.
13. Mamiya Y. Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus. Annual Review of Phytopathology. 1983; 21: 201–220. https://doi.org/10.1146/annurev.py.21.090183.001221
14. Mota M., Paulo C.V. Pine wilt disease in Portugal. Pine Wilt Disease. Springer, Tokyo, 2008; 33–39. https://doi.org/10.1007/978-4-431-75655-2_6
15. Ryss A. Y., Polyanina K. S., Popovichev B. G., Krivets S. A., Kerchev I. A. Plant host range specificity of Bursaphelenchus mucronatus Mamiya et Enda, 1979 tested in the laboratory experiments. Parazitologiya. 2018; 52: 32–40.
16. Nose M., Shiraishi S. Breeding for resistance to pine wilt disease in: Pine Wilt Disease. B. G. Zhao, K. Futai, J. R. Sutherland, Y. Takeuchi, eds. Springer Tokyo, 2008: 334–350. https://doi.org/10.1007/9784-431-75655-2_34
17. Ohba K., Nishimura K., Toda T., Tateyama T. Between mother tree variation in survival rate of pine seedlings after artificial inoculation with Bursaphelenchus xylophilus. Bulletin of the graduate school of social and cultural studies Kyushu university. 1977; 30: 69–70. [In Jap.]
18. Sutherland J. R., Ring F. M., Seed J. E. Canadian conifers as hosts of the pinewood nematode (Bursaphelenchus xylophilus): results of seedling inoculations. Scandinavian Journal of Forest Research. 1991; 6 (1-4): 209–216. https://doi.org/10.1080/02827589109382662
19. Wang Y., Jin M., Wang Y., Yang Y., Yu A. Infestation of pine seedlings (Pinus thunbergii Parl.) with pine wood nematode, Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) through needle leaves. Journal of Asia-Pacific Entomology. 2024; 27 (2): 102252. https://doi.org/10.1016/j.aspen.2024.102252
20. Wang Z., Calderon M. M., Carandang M. G. Effects of resin tapping on optimal rotation age of pine plantation. Journal of forest economics. 2006; 11 (4): 245-260. https://doi.org/10.1016/j.jfe.2005.10.001
Review
For citations:
Kulinich O.A., Arbuzova E.N., Kozyreva N.I., Chalkin A.A., Shchukovskaya A.G., Ryaskin D.I. Study of susceptibility of siberian pine (Pinus sibirica), scots pine (Pinus sylvestris) and siberian larch (Larix sibirica) seedlings to the pine wilt disease. Russian Journal of Parasitology. 2025;19(1):125-138. (In Russ.) https://doi.org/10.31016/1998-8435-2025-19-1-125-138
Views:
882
Email this article 