Histological examination of rabbit tissues and internal organs during experimental infection with Trichinella spiralis larvae
https://doi.org/10.31016/1998-8435-2024-18-4-398-409
Abstract
The purpose of the research is to conduct a histological examination of the tissues and organs of rabbits experimentally infected with Trichinella spiralis larvae.
Materials and methods. Necropsy was performed and organ and tissue samples were taken for histological studies 45 days after the experimental infection of White Giant rabbits with T. spiralis. The samples were fixed in 10% buffered formalin. Histological preparations: paraffin sections 4 μm thick, stained with hematoxylin and eosin.
Results and discussion. A complete histological examination of the dermis, muscles and internal organs in experimental trichinellosis was conducted. The effect of T. spiralis larvae on the body of rabbits and the pathological changes in organs and tissues were determined. Pathological changes of an inflammatory nature were established in the heart muscle, kidneys, lungs, small intestine, spleen and muscle tissue and the muscle layer of dermis with the presence of accumulation of lymphocytes and eosinophils. Colored illustrations of the obtained sections with a detailed description are provided.
About the Authors
F. I. Vasilevich
Federal State Budgetary Educational Institution of Higher Education “Moscow State Academy of Veterinary Medicine and Biotechnology – MVA named after K. I. Skryabin“
Russian Federation
Russian Federation
Fedor I. Vasilevich, Doctor of Veterinary Sciences, Academician of the Russian Academy of Sciences, Head of the Department of Parasitology and Veterinary and Sanitary Expertise
Researcher ID: K-9491-2015, Scopus ID: 57190309524
Moscow
M. V. Arisov
All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV” (VNIIP – FSC VIEV)
Russian Federation
Russian Federation
Michail V. Arisov, Doctor of Veterinary Sciences, Professor of the Russian Academy of Sciences, Head of the Laboratory of Ectoparasitosis, Head
Researcher ID: B-7834-2018, Scopus ID: 57207942094
Moscow
A. Yu. Vishnevskaya
Federal State Budgetary Educational Institution of Higher Education “Moscow State Academy of Veterinary Medicine and Biotechnology – MVA named after K. I. Skryabin“
Russian Federation
Russian Federation
Alena Yu. Vishnevskaya, Postgraduate student of the Department of Parasitology and Veterinary-Sanitary Expertise
Moscow
References
1. Britov V. A. Some distinctive features of natural and synanthropic trichinella. Wiadomości parazytologiczne. 1969; 15 (5-6): 555-560. (In Russ.)
2. Britov V. A. Causal agents of trichinellosis. Moscow: Nauka, 1982; 271. (In Russ.)
3. Garkavi B. L. Composition of potential hosts of Trichinella pseudospiralis. Parazitologija = Parasitology. 1974; VIII. 6: 489-493. (In Russ.)
4. Movsesyan S. O., Asatryan A. M. Study of developmental features of Trichinella spiralis Oven, 1932 and Trichinella pseudospiralis Garkavi, 1972. «Teoriya i praktika bor'by s parazitarnymi boleznyami»: materialy dokladov nauchnoy konferentsii = “Theory and practice of parasitic disease control”: materials of reports of scientific conference. 1999; 166-168. (In Russ.)
5. Akibekov O. S., Syzdykova A. S., Lider L. A., Zhumalin A. K.h., Baibolin Z. K., Zhagipar F. S.,Akanova Z. Zh., Ibzhanova A. A., Gajimuradova A. M. Hematological, biochemical, and serological parameters of experimentally infected rabbits with Trichinella nativa and Trichinella spiralis for early identification of trichinellosis. Veterinary World. 2022; 15 (9): 2285–2292. https://doi.org/10.14202/vetworld.2022.2285-2292
6. Jasmer D. P. Trichinella spiralis infected skeletal muscle cells arrest in G2/M and cease muscle gene expression. Journal of Cell Biology. 1993; 121 (4): 785-93. https://doi.org/10.1083/jcb.121.4.785
7. Kociecka W. Trichinellosis: human disease, diagnosis and treatment. Veterinary Parasitology. 2000; 93 (3-4): 365-83. https://doi.org/10.1016/s0304-4017(00)00352-6
8. Neghina R., Neghina A. M., Marincu I., Iacobiciu I. Reviews on trichinellosis (I): renal involvement. Foodborne Pathogens and Disease. 2011; 8 (2): 179- 188. https://doi.org/10.1089/fpd.2010.0704
9. Piergili Fioretti D., Moretti A., Marini C., Pasquali P., Morgante M., Polidori G. A. Experimental infection of rabbits and bird hosts with Trichinella britovi and Trichinella pseudospiralis: comparison of the biological characteristics. «Trichinellosis, ICT 9», P. G. Ortega, H. R. Gamble, F. Knapen, D. Wakelin (eds.). Mexico: CINVESTAV, 1996; 127-134.
10. Pozio E., La Rosa G., Rossi P., Murrel K. D. Biological characterization of Trichinella isolates from various host species and geographical regions. Journal of Parasitology. 1992; 78 (4): 647-653.
11. Pozio E. The broad spectrum of Trichinella hosts: From cold- to worm-blooded animals. Veterinary Parasitology. 2005; 132 (1-2): 3-11. https://doi.org/10.1016/j.vetpar.2005.05.024
12. Skrypec G., Nowazowski Z. Intensity of Trichinella spiralis invasion in the muscles of experimentally rabbits. Medycyna Weterynaryjna. 2010; 66 (2): 127-130.
13. Stewart G. L. Pathophysiology of the Muscle Phase. In book: Trichinella and Trichinosis. New: York Plenum Press, 1983; 241–264.
14. Wang J.-G., Mahmud S. A., Thompson J. A., Geng J.-G., Key N. S., Slungaard A. The principal eosinophil peroxidase product, HOSCN, is a uniquely potent phagocyte oxidant inducer of endothelial cell tissue factor activity: a potential mechanism for thrombosis in eosinophilic inflammatory states. Blood. 2006; 107 (2): 558-565. https://doi.org/10.1182/blood-2005-05-2152
15. Yan J., Huang S., Lu F. Galectin-Receptor Interactions Regulates Cardiac Pathology Caused by Trichinella spiralis Infection. Frontiers in Immunology. 2021; 12: 639260. https://doi.org/10.3389/fimmu.2021.639260
Review
For citations:
Vasilevich F.I., Arisov M.V., Vishnevskaya A.Yu. Histological examination of rabbit tissues and internal organs during experimental infection with Trichinella spiralis larvae. Russian Journal of Parasitology. 2024;18(4):398-409. (In Russ.) https://doi.org/10.31016/1998-8435-2024-18-4-398-409
Views:
1050
Email this article 