Identification of Dibothriocephalus nihonkaiensis plerocercoids from the autumn chum salmon Oncorhynchus keta of the Amur River basin by molecular datas
A. G. Dragomeretskaya,
Yu. I. Moskvina,
V. O. Kotova,
L. V. Butakova,
S. I. Gaer,
O. E. Trotsenko,
E. V. Podorozhnyuk https://doi.org/10.31016/1998-8435-2024-18-4-388-397
Abstract
The purpose of the research is determination of the species belonging to the Dibothriocephalus spp. plerocercoids from the autumn chum salmon Oncorhynchus keta of the Amur River basin based on the analysis of a gene fragment of the first cytochrome c oxidase (COX1) subunit of mitochondrial DNA.
Materials and methods. A total number of 61 salmon specimens (Oncorhynchus keta) caught in the Amur River and its tributaries were examined with helminthological analysis. DNA of Dibothriocephalus spp. was isolated from plerocercoids and afterwards amplified to detect mitochondrial COX1 partial gene of the D. nihonkaiensis. Products of amplification were sequenced via Sanger sequencing. Obtained nucleotide sequences were analyzed in BLAST.
Results and discussion. Type F plerocercoids were detected in nine specimens of O. keta. The sequenced fragments showed 99.31–100 % similarity with GenBank reference sequences of D. nihonkaiensis. The conducted research confirmed infestation with Dibothriocephalus nihonkaiensis (dyphyllobotriosis pathogen) of O. keta species caught in the Amur River in the territory of the Khabarovsk krai.
About the Authors
A. G. Dragomeretskaya
FBIS Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal service for surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Russian Federation
Russian Federation
Anna G. Dragomeretskaya, Candidate of Biological Sciences, Head of the Department of Natural Focal Infections
Researcher ID: LPP-3719-2024, Scopus ID: 56771412100
Khabarovsk
Yu. I. Moskvina
FBIS Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal service for surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Russian Federation
Russian Federation
Yulia I. Moskvina, Junior Researcher, Laboratory of Parasitology, Department of Natural Focal Infections
Khabarovsk
V. O. Kotova
FBIS Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal service for surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Russian Federation
Russian Federation
Valerya O. Kotova, Senior Researcher, Head of the Laboratory of Epidemiology and Prevention of Viral Hepatitis and AIDS
Researcher ID: B-9734-2018, Scopus ID: 7004305557
Khabarovsk
L. V. Butakova
FBIS Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal service for surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Russian Federation
Russian Federation
Ludmila V. Butakova, Researcher
Researcher ID: KVB-5728-2024, Scopus ID: 57209077159
Khabarovsk
S. I. Gaer
FBIS Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal service for surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Russian Federation
Russian Federation
Svetlana I. Gaer, Junior Researcher, Laboratory of Parasitology, Department of Natural Focal Infections
Khabarovsk
O. E. Trotsenko
FBIS Khabarovsk Research Institute of Epidemiology and Microbiology of the Federal service for surveillance on Consumers Rights Protection and Human Wellbeing (Rospotrebnadzor)
Russian Federation
Russian Federation
Olga E. Trotsenko, Doctor of Medical Sciences, Director
Scopus ID: 6602254191
Khabarovsk
E. V. Podorozhnyuk
Khabarovsk branch of Russian Federal Research Institute of Fisheries and Oceanography
Russian Federation
Russian Federation
Elena V. Podorozhnyuk, Head of the Salmon Laboratory
Khabarovsk
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Review
For citations:
Dragomeretskaya A.G., Moskvina Yu.I., Kotova V.O., Butakova L.V., Gaer S.I., Trotsenko O.E., Podorozhnyuk E.V. Identification of Dibothriocephalus nihonkaiensis plerocercoids from the autumn chum salmon Oncorhynchus keta of the Amur River basin by molecular datas. Russian Journal of Parasitology. 2024;18(4):388-397. (In Russ.) https://doi.org/10.31016/1998-8435-2024-18-4-388-397
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