Acute toxicity of a combined fipronil and pyriproxyfen shampoo

Abstract

The purpose of the research is to study the acute oral and dermal toxicity of a veterinary drug (fipronil, pyriproxyfen) in mice and rats.
Materials and methods. The studies were performed according to the Methodical Guidelines of the State Pharmacological Committee in the vivarium of the VNIIP – FSC VIEV from February to April 2021. White outbred male mice and male rats were used as a test system to study toxicological characteristics of the study drug. The veterinary drug Insektal Shampoo is a topical solution that contains fipronil and pyriproxyfen as active ingredients. In studying the acute oral toxicity and acute dermal toxicity of the study drug on laboratory animals, established procedures were used.
Results and discussion. The median lethal dose (LD₅₀) was 12 500 (11,532÷13,468) mg/kg when administered to the white male mice intragastrically as per Miller-Tainter method. According to the generally accepted hygiene classification, the drug was classified as hazard class 4 according to GOST 12.1.007-76. For the white male rats, LD₅₀ was more than 20,800 mg/ kg (hazard class 4). When applied to intact rat skin, LD₅₀ was more than 10 400 mg/kg, which corresponds to hazard class 4.