Antimitotic effects of Cysticercus tenuicollis protoscolexes extract at administration to mice and their negative consequences for organism

The purpose of the research is studying of Cysticercus tenuicollis protoscolexes extract effects on cell division at different routes of administration to mice and evaluation of the associated negative effects.
Materials and methods. C. tenuicollis were obtained from spontaneously infected sheep in Kabardino-Balkarian Republic. C. tenuicollis protoscolexes were washed, crushed and homogenized. Protein extraction was performed with phosphate buffered saline pH 7.2–7.4. C. tenuicollis extract was administered intraperitoneally and intravenously to mice males at the dose level of 80 μg protein/animal. The control group of mice was intravenously injected with 0.1 ml of saline. At hours 3; 6; 24 and 48 post extract administration mice were euthanized. Bone marrow samples were taken from experimental and control mice for preparation of microscopic preparations to assess mitotic activity in a given cell population. The mitotic index was determined, all stages of mitosis were recorded. At the above time points blood samples were taken from mice to determine the main hematological parameters post intravenous and intraperitoneal administration of C. tenuicollis extract. The main hematological parameters of mice were determined using hematological analyzer MicroCC-20 Plus (High Technology, Inc. (USA)); leukocyte formula – by the generally accepted method. Samples of liver, kidneys, spleen, mesenteric lymph nodes and testes were taken from experimental and control animals for macroscopic and microscopic studies.
Results and discussion. C. tenuicollis protoscolices extract leads to inhibition of cell division in the population bone marrow and testes cells in mice when administered intravenously and intraperitoneally at the dose level of 80 μg/animal manifested in accumulation of metaphases and decrease of other stages. At both routes of administration a decrease in leukocyte counts was noted. The observed microscopic changes in testes, spleen and lymph nodes either reflect the consequences of extract antimitotic effect or the immune response to the administration of C. tenuicollis extract.

1. Gordina E. V. Caryopathic and pathomorphological effects of metabolic products of Fasciola hepatica and Bacillus subtilis: authoref. dis. … cand. vet. sci. Perm, 2016; 24. (In Russ.)

2. Investigation of effects of trichinella of different species and development stages on mitosis in a host. Meditsinskaya parazitologiya i parazitarnyye bolezni = Medical Parasitology and Parasitic Diseases. 2020; 2: 9-14. (In Russ.) https://doi.org/10.33092/0025-8326mp2020.2.09-14

3. Koveshnikova E. I., Novik T. S., Napisanova L. A., Chukina S. I., Rudneva O. V. Evaluation of karyopathic effects and general safety of Trichinella spiralis and Echinococcus multilocularis extracts in mice. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2020; 14 (4): 90–98. (In Russ.). https://doi.org/10.31016/1998-8435-2020-14-4-90-98

4. Novik T. S., Koveshnikova E. I., Chukina S. I., Napisanova L. A., Andreyanov O. N., Arkhipov I. A., Kurochkina K. G. Effects of Trichinella spiralis and Echinococcus multilocularis extracts after single and multiple injections on mitosis and hematological and biochemical parameters of mice. Rossiyskiy parazitologicheskiy zhurnal = Russian Journal of Parasitology. 2022; 16 (4): 411–420. (In Russ.). https://doi.org/10.31016/1998-8435-2022-16-4-411-420

5. Maksimenko S. N. Effects of Trichinella spiralis and Trichinella pseudospiralis infection on mouse bone marrow cell mitosis and manifestation of the albendazole antimitotic effect. Proceedings of the All-Russian Institute of Helminthology named after K. I. Skryabin. 2007; 43. 166-175. (In Russ.)

6. Order by the Russian Federation Ministry of Health No. 708n dated 23/08/2010 on Laboratory Practice Rules. (In Russ.)

7. Order by the USSR Ministry of Health No. 755 dated 12/08/1977 on Guidelines for Work Using Experimental Animals. (In Russ.)

8. Order by the USSR Ministry of Health No. 1045- 73 dated April 6, 1973 on Sanitary Rules for Arrangement, Equipment and Maintenance of Experimental Biological Clinics (Vivariums)".

9. Sivkova T. N. Caryopathic effects of Anisakis larvae products on somatic and germ cells of laboratory rats after oral administration. Materials of the Scientific Conference "Theory and practice of parasitic disease control". М., 2009; 10: 366-370. (In Russ.)

10. Sogrina A. V. Dirofilariosis of service dogs in the Perm region (distribution, serological monitoring, caryopathic effect of Dirofilaria immitis antigens and antiparasitic drugs): authoref. dis. … cand. biol. sci. M., 2017; 26. (In Russ.)

11. European Convention for Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes (ETS 123). Strasbourg, 1986.

12. Ford C. E., Hamerton J. L. A colchicines, hypotonic citrate squach sequence for mammalian chromosomes. Stain Technol. 1956; 31 (6): 247-251.