vniigisРоссийский паразитологический журналRussian Journal of Parasitology1998-84352541-7843ВНИИП – филиал ФГБНУ ФНЦ ВИЭВ РАН10.12737/20061vniigis-337Research ArticleЭПИЗООТОЛОГИЯ, ЭПИДЕМИОЛОГИЯ И МОНИТОРИНГ ПАРАЗИТАРНЫХ БОЛЕЗНЕЙEPIZOOTOLOGY, EPIDEMIOLOGY AND MONITORING OF PARASITIC DISEASESMELANOIDES KAINARENSIS НОВЫЙ ПРОМЕЖУТОЧНЫЙ ХОЗЯИН ТРЕМАТОДЫ PHILOPHTHALMUS LUCIPETUS (TREMATODA, PHILOPHTHALMIDAE)MELANOIDES KAINARENSIS NEW INTERMEDIATE HOST OF TREMATODES PHILOPHTHALMUS LUCIPETUS (TREMATODA, PHILOPHTHALMIDAE)ШакарбаевУ. А.ShakarbaevU. A.

Ул. Богишамол 232, Ташкент, 100053, Узбекистан, ushakarbaev@mail.ru

232 Bogishamol Str., Tashkent 100053, Uzbekistan, E-mail: ushakarbaev@mail.ru

ushakarbaev@mail.ruАкрамоваФ. Д.AkramovaF. D.

Ул. Богишамол 232, Ташкент, 100053, Узбекистан, ushakarbaev@mail.ru

232 Bogishamol Str., Tashkent 100053, Uzbekistan, E-mail: ushakarbaev@mail.ru

ushakarbaev@mail.ruАзимовД. А.AzimovD. A.

Ул. Богишамол 232, Ташкент, 100053, Узбекистан, ushakarbaev@mail.ru

232 Bogishamol Str., Tashkent 100053, Uzbekistan, E-mail: ushakarbaev@mail.ru

ushakarbaev@mail.ruИнститут генофонда растительного и животного мира Академии Наук Республики УзбекистанУзбекистанInstitute of Gene Pool of Plants and Animals, Academy of Sciences of the Republic of UzbekistanUzbekistan201620122016362183191Copyright © Шакарбаев У.А., Акрамова Ф.Д., Азимов Д.А., 20162016Шакарбаев У.А., Акрамова Ф.Д., Азимов Д.А.Shakarbaev U.A., Akramova F.D., Azimov D.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://vniigis.elpub.ru/jour/article/view/337

Цель исследования – изучение зараженности моллюсков MelanoideskainarensisStarobogatovetIzzatullaev, 1980 церкариями трематоды Philophthalmuslucipetus (Rudolphi, 1819) в естественных условиях Узбекистана.

Материалы и методы

Материалы и методы. Исследованы церкарии и партениты Ph. lucipetus, из моллюсков M. kainarensis из теплого источника Бошховуз Самаркандской области Узбекистана. Морфологические и биологические исследования осуществлены известными паразитологическими методами.

Результаты и обсуждение

Результаты и обсуждение. При исследовании 2801 экз. M. kainarensis оказались зараженными церкариями и партенитами рода PhilophthalmusLooss, 1899, которые были идентифицированы и отнесены к виду Ph. lucipetus. Собранные церкарии и адолескарии использованы для экспериментального заражения Anseranserdom., Anasplatyrhynchоsdom. и Gallusgallusdom. Взрослые паразиты, выделенные из конъюнктивального мешка зараженных птиц были определены как Philophthalmuslucipetus. Это первый случай, подтверждающий участие M. kainarensis в качестве промежуточного хозяина указанной трематоды.

Objective of research

Objective of research: to study the infection level of molluscs Melanoides kainarensis Starobogatov et Izzatullaev, 1980 with cercariae of trematode Philophthalmus lucipetus (Rudolphi, 1819) under conditions of Uzbekistan.

Materials and methods

Materials and methods: Cercariae and parthenitae of Ph. lucipetus of  molluscs M. kainarensis from the warm spring Boshkhovuz of Samarkand region, Uzbekistan were investigated.  Morphological and biological studies were carried out by well-known standard parasitological methods.

Results and discussion

Results and discussion: The research revealed that 2801 specimens of M. kainarensis were infected with cercariae and parthenitae of Philophthalmus Looss, 1899, which were identified as Ph. lucipetus. The collected cercariae and adolescariae were used for the experimental infestation with Anser anser dom., Anas platyrhynchos dom. and Gallus gallus dom. Adult parasites isolated from conjunctival sacs of infected birds were identified as Philophthalmus lucipetus. That was the first evidence for the role of M. kainarensis as the intermediate host for these trematodes.

Melanoides kainarensisPhilophthalmus lucipetusпартенитыцеркарииадолескариитрематодыптицымоллюскиMelanoides kainarensisPhilophthalmus lucipetusparthenitaecercariaeadolescariaetrematodebirdsmollusksReferencesАтаев Г.Л. Влияние температуры на развитие и биологию редий и церкарий Philophthalmus rhionica (Trematoda). // Паразитология, 1991. 25 (4): С. 349 359.Astaf'ev B.A., Petrov O.E. Evolutionary and genetic theory of parasitism. Uspehisovremennojbiologii [Advances in modern biology], 1992, vol.112, no. 2, pp. 163-175. (In Russian)Галактионов К.В., Добровольский А.А. Гермафродитное поколение трематод. Ленинград, Наука, 1987. 193 с.Berezhko V.K., Khaydarov V.K., Dahno N.S., Shkurka E.P. Immunochemical analysis of somatic extract from mature Dirofilariaimmitis. Mater. dokl. nauch. konf. Vseros. o-va gel’mintol. RAN «Teoriya i praktika bor’by s parazitarnymi boleznyami». [Proc. of sci. conf. of All-Russian Society of Helminthol. RAS «Theory and practice of the struggle against parasitic diseases]. M., 2008, i. 9, pp. 69-73. (In Russian)Галактионов К.В., Добровольский А.А. Происхождение и эволюция жизненных циклов трематод. Санкт Петербург, Наука, 1998. 403 с.Berezhko V.K., Napisanova L.A., Medvedev A.Yu.,Khaydarov K.A., Shinkarenko A.N., Kolesnikov P.V. Physicochemical and immunodiagnostic characteristics of whole and fractionated somatic extract of Dirofilariaimmitis. Doklady Rossel’hozakademii. [Proceedings of the Russian Academy of Agricultural Sciences], 2014, no. 1, pp. 71-74. (In Russian)Гинецинская Т. А. Трематоды, их жизненные циклы, биология и эволюция. Ленинград, Наука, 1968. 411с.Beskrovnaya Yu.G., Nagornyi S.A. Identification of microfilaria Dirofilaria spp. by polymerase chain reaction (PCR). Mater. dokl. nauch. konf. Vseros. o-va gel’mintol. RAN «Teoriya i praktika bor’by s parazitarnymi boleznyami». [Proc. of sci. conf. of All-Russian Society of Helminthol., RAS «Theory and practice of the struggle against parasitic diseases]. M., 2008, pp. 73-75. (In Russian)Жадин В.И. Моллюски пресных и солоноватых вод СССР. Определители по фауне СССР. АН СССР, Москва, Ленинград, 1952. 374 с.Beskrovnaya Ju.G., Nagornyi S.A., Vaserin Yu.I. Efficacy evaluation of diagnosis of dirofilarias is indogsusing the Dirofilariarepens somatic antigen. Mater. dokl. nauch. konf. Vseros. o-vagel’mintol. RAN «Teoriya i praktika bor’by s parazitarnymi boleznyami». [Proc. of sci. conf. of All-Russian Society of Helminthol., RAS «Theory and practice of the struggle against parasitic diseases]. M., 2009, i. 10, pp. 61-63. (In Russian)Красная книга республики Узбекистан. Животные. Ташкент, Chinor ENK, 2: 2009. С. 40 41.Krivorotova E.Yu. Biologicheskie aspekty dirofilyarioza v ryade sub’ektov Rossiyskoy Federatsii. Dis. … kand. biol. nauk [Biological aspects of dirofilariasis in a number of constituent territories of the Russian Federation. PhD diss... biol. sci.]. M., 2015. 141 p. (In Russian)Лихарев И.М., Старобогатов Я.И. Материалы к фауне моллюсков Афганистана. Сб. «Моллюски и их роль в биоценозах и формировании фаун». // Труды Зоологического института АН СССР, Ленинград, Наука, 42: 1967. С. 159 198.Medvedev A.Yu. Rasprostranenie dirofilyarioza sobak v Krasnodarskom krae i razrabotka ego diagnostiki immunofermentnoy reakciey. Diss. … kand. Biol. Nauk [Prevalence of dirofilariasis in dogs in Krasnodar Krai and development of diagnostic techniques using an immunoenzymatic reaction. PhD diss... biol. sci.]. M., 2007.137 p. (In Russian)Смогоржевская А.А. Гельминты водоплавающих и болотных птиц фауны Украины. Киев, Наукова думка, 1976. 414 с.Sergiev V.P., Supryaga V.G., Darchenkova N.N., Zhukova L.A., Ivanova T.N. Human dirofilariasis in Russia. Rossiyskiy parazitologicheskiy zhurnal [Russian Journal of Parasitology], 2012, no. 4, pp. 60-64. (In Russian)Старобогатов Я.И., Иззатуллаев З.И. Моллюски семейства Melanoididae (Gastropoda, Pectinibanchia) Средней Азии и сопредельных территорий. // Зоологический журнал, Наука, LIX (1): 1980. С. 23-31.Sergiev V.P., Supryaga V.G., Bronshtein A.M., Ganushkina L.A.,Rakova V.M., Morozov E.N., Fedyanina L.V., Frolova A.A., Morozova L.F., Ivanova I.B., Darchenkova N.N., Zhukova L.A. Results of the study on dirofilariasis in Russia. Meditsinskaya parazitologiya i parazitarnye bolezni [Medical Parasitology and Parasitic Diseases], 2014, no. 3, pp. 3-9. (In Russian)Тихомиров И.А. Жизненный цикл Philophthalmus rhionica sp. nov. (Trematoda: Philophthalmidae). Автореф. дис. ... канд. биол. наук. Ленинград, 1980. 20 с.Fis’ko M.A., Firsov N.F. Dirofilyarioz [Dirofilariasis], Rostov-onDon, 2006. 110 p. (In Russian)Alicata J.E. Life cycle and development stages of Philophthalmus gralli in intermediate and final hosts. Journal of Parasitology., 48: 1962. P. 47 54.Khaydarov K.A., Berezhko V.K. Antigenic spectrum of somatic extracts from mature Setaria and Dirofilaria. Rossiyskiy parazitologicheskiy zhurnal [Russian Journal of Parasitology], 2009, no 1, pp. 68–74. (In Russian)Abdul-Salam J., Sreelatha B.S., Ashkanani H. The eye fluke Philophthalmus hegeneri (Digenea: Philophthalmidae) in Kuwait Bay. Kuwait Journal of Science Eng., 31(1): 2004. P. 119 133.Akao N. Human dirofilariasis in Japan. Trop Med Health, 2011, vol. 39, no. 1. Suppl. 2, pp. 65-71.Greve J.H., Harrison G.J. Conjunctivitis caused by eye flukes in captive-reared ostriches. Journal of the American Veterinary Association, 177: 1980. P. 909 910.Atwell R.B., Sheridan A.B., Baldock F.C. An evaluation of the Dirochek test for detection of Dirofilariaimmitis antigen in dogs. Aust. Vet. J., 1988, vol. 65, no. 5, pp. 161-162.Kanev I., Nollen P.M., Vassilev I., Radev V., Dimitrov V. Redescription of Philophthalmus lucipetus (Rudolphi, 1819) (Trematoda: Philophthalmidae) with a discussion of its identity and characteristics. Annalen des Naturhistorisches Museum Wien 94/95B, 1993. P. 11 34.Capron A., Brygoo E.R., Afchain D. Apport de letude de la structure antigenique a la phylogenie des Helminthes. Bull. Mus. Nat. hist. natur. Zoo, 1972, vol. 55, pp. 877-885.Literák I., Heneberg P., Sitko J., Wetzel E.J., Cardenas Callirgos J.M., Сapek M., Valle Basto D., Papousek I. Eye trematode infection in small passerines in Peru caused by Philophthalmus lucipetus, an agentwith a zoonotic potential spread by an invasive freshwater snail. Parasitology International, 62: 2013. P. 390 396.Collins G.H., Pope S.E. An evaluation of an ELISA test for the detection of antigens of Dirofilariaimmitis. Aust. Vet. J., 1987, vol. 64, no. 10, pp. 318-319.Mukaratirwa S., Hove T., Cindzi Z.M., Maononga D.B., Taruvinga M., Matenga E. First report of a field outbreak of the oriental eye-fluke, Philophthalmus gralli (Mathis & Leger 1910), in commercially reared ostriches (Struthio camelus) in Zimbabwe. Onderstepoort Journal of Veterinary Research, 72: 2005. P. 203 206.Gioia G., Lecová L., Genchi M. et al. Highly sensitive multiplex PCR for simultaneous detection and discrimination of Dirofilariaimmitis and Dirofilariarepens in canine peripheral blood. Vet. Parasitol. , 2010, vol. 172, no. 1-2, pp. 160-163.Pinto H.A. Infecҫão natural de Melanoides tuberculata (Mollusca: Thiaridae) por Centrocestus formosanus (Trematoda: Heterophyidae) e por Philophthalmus gralli (Trematoda: Philophthalmidae) no Brasil. Universidade Federal de Minas Gerais Instituto de Ciencias Biologicas Belo Horizonte, 2009. P. 55 67.Latrofa M.S., Dantas-Torres F., Annoscia G. et al. A duplex real-time polymerase chain reaction ssay for the detection of and differentiation between Dirofilariaimmitis and Dirofilariarepens in dogs and mosquitoes. Vet. Parasitol., 2012, vol. 185, no.2-4, pp. 181-185.Radev V., Kanev I., Fried B. Comments on eye-flukes (Philophthalmidae Looss, 1819) in the genera Philophthalmus and Natterophthalmus, with a re-designation of the type-species of Natterophthalmus. Zootaxa, 1223: 2006. P. 19 22.Lee A.C., Bowman D.D., Lucio-Forster A. et al. Evaluation of a new in-clinic method for the detection of canine heartworm antigen. Vet. Parasitol., 2011, vol. 177, no. 3-4, pp. 387-391.West A.F. Studies on the biology of Philophthalmus gralli Mathis and Leger, 1910 (Trematoda: Digenea). American Midland Naturalist, 66: 1961. P. 363 383.Mc Call J.W., Genchi C., Kramer L.H. et al. Heartworm disease in animals and humans. Adv. Parasitol., 2008, vol. 66, pp. 193-285.Morchon R., Carreton E., Gonzalez-Miguel J., Mellado-Hernandez I. Heartworm Disease (Dirofilariaimmitis) and Their Vectors in Europe — New Distribution Trends. Front. Physiol., 2012, vol. 3, Art. 196.Morchon R., Carreton E., Gonzalez-Miguel J., Mellado-Hernandez I. Heartworm Disease (Dirofilariaimmitis) and Their Vectors in Europe — New Distribution Trends. Front. Physiol., 2012, vol. 3, Art. 196.Renaud F. Biodiversite, genetiqueet evolution dans les systemeshotes-parasites. Bull. Soc. Zool. Ff., 1992, vol. 117, no.1, pp. 109-115.Renaud F. Biodiversite, genetiqueet evolution dans les systemeshotes-parasites. Bull. Soc. Zool. Ff., 1992, vol. 117, no.1, pp. 109-115.Rivasi F., Boldorini R., Criante P. et al. Detection of Dirofilaria (Nochtiella) repens DNA by polymerase chain reaction in embedded paraffin tissues from two human pulmonary locations. APMIS, 2006, vol. 114, no. 7-8, pp. 567-574.Rivasi F., Boldorini R., Criante P. et al. Detection of Dirofilaria (Nochtiella) repens DNA by polymerase chain reaction in embedded paraffin tissues from two human pulmonary locations. APMIS, 2006, vol. 114, no. 7-8, pp. 567-574.Sun M., Zhuo W., Guo S. et al. Serological survey of canine dirofilariosis in Chongqing, Kunming, Nanchang, Fuzhou, Guangzhou, Shenzhen, and Nanning in Southern China. Vet. Parasitol., 2012, vol. 185, no. 2-4, pp. 225-228.Sun M., Zhuo W., Guo S. et al. Serological survey of canine dirofilariosis in Chongqing, Kunming, Nanchang, Fuzhou, Guangzhou, Shenzhen, and Nanning in Southern China. Vet. Parasitol., 2012, vol. 185, no. 2-4, pp. 225-228.Weil G.J., Malane M.S, Powers K.G. Detection of circulating parasite antigens in canine dirofilariasis by counterimmunoelectrophoresis. Am. J. Trop. Med. Hyg., 1984., vol. 33, no. 3, pp. 425-430.Weil G.J., Malane M.S, Powers K.G. Detection of circulating parasite antigens in canine dirofilariasis by counterimmunoelectrophoresis. Am. J. Trop. Med. Hyg., 1984., vol. 33, no. 3, pp. 425-430.

The authors declare that there are no conflicts of interest present.