vniigisРоссийский паразитологический журналRussian Journal of Parasitology1998-84352541-7843ВНИИП – филиал ФГБНУ ФНЦ ВИЭВ РАН10.12737/23078vniigis-312Research ArticleБИОХИМИЯ, БИОТЕХНОЛОГИЯ И ДИАГНОСТИКАBIOCHEMISTRY, BIOTECHNOLOGY AND DIAGNOSTICSНУКЛЕОЗИДДИФОСФАТАЗЫ ЦЕСТОД BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE)NUCLEOSIDE-DIPHOSPHATASE OF CESTODE BOTHRIOCEPHALUS SCORPII (CESTODA: BOTHRIOCEPHALIDAE)БуренинаЭ. А.BureninaE. A.

690022, г. Владивосток, пр. 100-летия Владивостока, 159

690022, Vladivostok, 159 Prosp.100-letiya Vladivostoka

burenina@ibss.dvo.ruБиолого-почвенный институт ДВО РАНРоссияInstitute of Biology and Soil Sciences, Far East Branch of RASRussian Federation201615122016384527532Copyright © Буренина Э.А., 20162016Буренина Э.А.Burenina E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vniigis.elpub.ru/jour/article/view/312

Цель исследования — изучение активности и свойств нуклеозиддифосфатазы (НДФаза) у цестод Bothriocephalus scorpii.

Материалы и методы

Материалы и методы. Цестод гомогенизировали с 10 объемами среды выделения. НАДазы определяли в митохондриях и микросомах с субстратами (ИДФ, ГДФ, УДФ). Неорганический фосфор определяли по Кочетову (1980). Было испытано действие 10 антигельминтных препаратов на активность НДФазы.

Результаты и обсуждение

Результаты и обсуждение. Обнаружили, что митохондриальные и микросомальные фракции цестод B.scorpii обладают нуклеозиддифосфатазной активностью. Активность фермента зависит от субстратов и ионов Mg2+. Изучено влияние различных эффекторов и ионов (Са2+, Mn2+, Zn2+) на энзимную активность. Эффект 10 антгельминтных препаратов на активность НДФазы был изучен. Эффективными препаратами являются битионол и трихлорофен.

Objective of research

Objective of research: To study the activities and properties of nucleoside- diphosphatase (NDPase) in cestode Bothriocephalus scorpii.

Materials and methods

Materials and methods: Cestodes were homogenized with 10 vol. of extraction medium. NDPase was detected in mitochondria and microsomes with substrates (IDP, GDP, UDP). Inorganic phosphorus was determined by the method of Kochetov (1980). The effects of 10 anthelmintic drugs on the activity of NDPase were studied.

Results and discussion

Results and discussion: It was found that the mitochondrial and microsomal fractions of cestodes B. scorpii have nucleoside diphosphatase activity.

The activity of nucleoside diphosphatase depends on substrates and Mg2+ ions. The impact of various effectors and ions (Ca2+, Mn2+, Zn2+) on enzyme activity was determined.

Effects of 10 anthelmintic drugs on activity of nucleoside diphosphatase were studied. The anthelmintics Bitionol and Trichlorophen have been proved effective.

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The authors declare that there are no conflicts of interest present.