vniigisРоссийский паразитологический журналRussian Journal of Parasitology1998-84352541-7843ВНИИП – филиал ФГБНУ ФНЦ ВИЭВ РАНvniigis-285Research ArticleБИОХИМИЯ, БИОТЕХНОЛОГИЯ И ДИАГНОСТИКАBIOCHEMISTRY, BIOTECHNOLOGY AND DIAGNOSTICSБИОИНФОРМАЦИОННАЯ ОЦЕНКА РНК-ЗАВИСИМОЙ РНК-ПОЛИМЕРАЗЫ ГРИБА-паразита Metarhizium anisopliaeA BIOINFORMATICS CHARACTERIZATION OF THE RNA-DEPENDENT RNA POLYMERASE FROM THE PARASITIC FUNGI, Metarhizium anisopliaeВЕЙЕРК. Н.WEIRC. N.

1G Ройал Парад, Парквилл 3052, Виктория

Парквилл 3010, Виктория

M. Mol. Biol. and Ph. D candidate 

1G Royal Parade, Parkville 3052, Victoria

Department of Medical Biology

Parkville 3010, Victoria

weir.c@wehi.edu.auРОНГЭ.EWONGE.

Хинкстон, Кембриджшир CB10 1SD

Ph. D. 

Cambridgeshire CB10 1SD

КИНГГ. Ф.KINGG. F.

кандидаты биологических наук 

Св. Лючия 4072, Брисбейн, Квинслэнд

Ph. D. 

St. Lucia 4072, Brisbane, Queensland

Институт Медицинских Исследований Уолтера и Элизы Холл; Мельбурнский университет, Отдел Медицинской БиологииАвстралияWalter and Eliza Hall Institute of Medical Research; University of MelbourneAustraliaЕвропейский институт биоинформатики (EMBL-EBI), Кампус "Wellcome Trust Genome"ВеликобританияEuropean Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, HinxtonUnited KingdomИнститут молекулярной биологии, Университет КвинслендаАвстралияInstitute for Molecular Bioscience, University of QueenslandAustralia201414052016045563Copyright © ВЕЙЕР К., РОНГ Э., КИНГ Г., 20162016ВЕЙЕР К., РОНГ Э., КИНГ Г.WEIR C., EWONG E., KING G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vniigis.elpub.ru/jour/article/view/285

Metarhizium anisopliae представляет собой энтомопатогенный гриб, паразитирующий на насекомых, включая тех, которые являются переносчиками инвазии, в том числе, зараженных малярийными паразитами москитов Anopheles. В качестве потенциально новой стратегии контроля переносчиков при исследовании M. anisopliae предлагается использовать биоинженерный метод. Однако, многие грибы-паразиты содержат ген РНК- зависимой РНК-полимеразы (RdRp), который служит защитой от трансгенов. Дана характеристика гена RdRp гриба M. anisopliae, включая его строение, представленное во взаимосвязи с окружающими его генами, и генетическое моделирование, основанное на изучении RdRp белка гриба Neurospora crassa. 

Metarhizium anisopliae is an entomopathogenic organism that is parasitic towards insects, including those that serve as vectors for human disease, including malaria parasite infected Anopheles mosquitoes. Directed bioengineering of parasitic M. anisopliae has been proposed as a potentially novel vector control strategy. However, many parasitic fungi posses a RNA-dependent RNA polymerase (RdRp) gene that serves as a defence against transgenes. Here we characterize the RdRp gene of M. anisopliae, including the context of the gene in relation to surrounding genes and theoretical modeling based on the RdRp enzyme of Neurospora crassa. 

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The authors declare that there are no conflicts of interest present.