vniigisРоссийский паразитологический журналRussian Journal of Parasitology1998-84352541-7843ВНИИП – филиал ФГБНУ ФНЦ ВИЭВ РАН10.31016/1998-8435-2024-18-4-449-462vniigis-1242Research ArticleЛЕЧЕНИЕ И ПРОФИЛАКТИКАTREATMENT AND PREVENTIONНегативная кросс-резистентность к хлорфенапиру у особей пиретроид-устойчивой популяции комнатной мухиNegative cross-resistance to chlorfenapyr in pyrethroid-resistance house flieshttps://orcid.org/0000-0003-0872-8509СиливановаЕ. А.SilivanovaE. A.

Силиванова Елена Анатольевна, кандидат биологических наук, ведущий научный сотрудник лаборатории молекулярной биологии и биотехнологии насекомых

Researcher ID: C-8266-2015, Scopus ID: 57203024713

Тюмень 

Elena A. Silivanova, candidate of Biological Sciences, Leading Researcher, Laboratory of Molecular Biology and Biotechnology of Insects

Researcher ID: C-8266-2015, Scopus ID: 57203024713 

Tyumen 

sylivanovaea@mail.ruhttps://orcid.org/0000-0002-3194-873XКинарейкинаА. Г.KinareikinaA. G.

Кинарейкина Анна Григорьевна, аспирант, младший научный сотрудник лаборатории молекулярной биологии и биотехнологии насекомых

Researcher ID: AFK-9864-2022, Scopus ID: 58076407400 

Тюмень 

Anna G. Kinareikina, postgraduate student, junior researcher at the Laboratory of Molecular Biology and Biotechnology of Insects

Researcher ID: AFK-9864-2022, Scopus ID: 58076407400 

Tyumen 

kinareickina@yandex.ruhttps://orcid.org/0009-0005-4021-2619НурисламоваА. Р.NurislamovaA. R.

Нурисламова Алина Райханжановна, лаборант-исследователь лаборатории молекулярной биологии и биотехнологии насекомых

Тюмень 

Alina R. Nurislamova, laboratory research assistant in the laboratory of molecular biology and insect biotechnology 

Tyumen 

lfeltnvbh@mail.ruhttps://orcid.org/0000-0002-3926-4754МельничукА. Д.MelnichukA. D.

Мельничук Анастасия Дмитриевна, младший научный сотрудник лаборатории молекулярной биологии и биотехнологии насекомых

Researcher ID: HJG-5175-2022 

Тюмень 

Anastasia D. Melnichuk, junior Researcher, Laboratory of Molecular Biology and Biotechnology of Insects 

Researcher ID: HJG-5175-2022 

Tyumen 

melnichukad1999@gmail.comhttps://orcid.org/0000-0002-9688-5207МаслаковаК. Ю.MaslakovaK. Yu.

Маслакова Ксения Юрьевна, аспирант, младший научный сотрудник лаборатории молекулярной биологии и биотехнологии насекомых

Тюмень 

Kseniya Yu. Maslakova, postgraduate student, Junior Researcher, Laboratory of Molecular Biology and Biotechnology of Insects

Tyumen 

k.y.maslakova@gmail.comhttps://orcid.org/0000-0002-7546-485XЯнгироваЛ. Я.YangirovaL. Ya.

Янгирова Лиана Януровна, аспирант, младший научный сотрудник лаборатории молекулярной биологии и биотехнологии насекомых

Researcher ID: HHN-5767-2022 

Тюмень 

Liana Ya. Yangirova, postgraduate student, junior researcher at the Laboratory of Molecular Biology and Biotechnology of Insects

Researcher ID: HHN-5767-2022 

Tyumen 

lianayangirova137@gmail.comhttps://orcid.org/0000-0003-3607-3706КрестоношинаК. С.KrestonoshinaK. S.

Крестоношина Ксения Сергеевна, зав. лабораторией молекулярной биологии и биотехнологии насекомых 

Researcher ID: AFM-4923-2022 

Тюмень 

Kseniya S. Krestonoshina, head of the Laboratory of Molecular Biology and Biotechnology of Insects 

Researcher ID: AFM-4923-2022 

Tyumen 

krutko.k.s@hotmail.comВсероссийский научно-исследовательский институт ветеринарной энтомологии и арахнологии – филиал Федерального государственного бюджетного учреждения науки Федерального исследовательского центра Тюменского научного центра Сибирского отделения Российской академии наук (ВНИИВЭА – филиал ТюмНЦ СО РАН)РоссияAll-Russian Scientific Research Institute of Veterinary Entomology and Arachnology – Branch of Federal State Institution Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences (ASRIVEA – Branch of Tyumen Scientific Centre SB RAS)Russian Federation202402122024184449462Copyright © Силиванова Е.А., Кинарейкина А.Г., Нурисламова А.Р., Мельничук А.Д., Маслакова К.Ю., Янгирова Л.Я., Крестоношина К.С., 20242024Силиванова Е.А., Кинарейкина А.Г., Нурисламова А.Р., Мельничук А.Д., Маслакова К.Ю., Янгирова Л.Я., Крестоношина К.С.Silivanova E.A., Kinareikina A.G., Nurislamova A.R., Melnichuk A.D., Maslakova K.Y., Yangirova L.Y., Krestonoshina K.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vniigis.elpub.ru/jour/article/view/1242

Цель исследования – оценить чувствительность к дельтаметрину и про-инсектициду хлорфенапиру у природной популяции Musca domestica L. в сравнении с лабораторной культурой Lab UF и установить возможный механизм кросс-резистентности к хлорфенапиру.Материалы и методы. Исследование выполнено на имаго лабораторной культуры Lab UF и природной популяции Nik комнатной мухи M. domestica, собранной в животноводческом хозяйстве Тюменской области, где длительное время применяли пиретроидные инсектициды. Токсичность пиретроида дельтаметрина (Дельцид, 4 %) и пиррола хлорфенапира (Пирафен КЭ, 360 г/л) оценивали методом группового кормления взрослых особей. На основании результатов токсикологических опытов рассчитывали летальные концентрации инсектицидов методом пробит-анализа и показатель резистентности. Для выяснения возможного механизма кросс-резистентности к хлорфенапиру у популяции Nik M. domestica определяли уровень активности основных ферментов детоксикации в зависимости от пола насекомых. Методом секвенирования по Сэнгеру выполнено исследование на наличие kdr-мутации, обеспечивающей резистентность к пиретроидам.Результаты и обсуждение. Установленные летальные концентрации инсектицидов и расчет показателей резистентности продемонстрировали средний уровень резистентности к дельтаметрину и высокую чувствительность к хлорфенапиру популяции Nik. Обнаружено статистически значимое увеличение активности монооксигеназ в 2,25–4,36 раза, глутатион-S-трансферазы в 2,02–2,18, ацетилхолинэстеразы в 1,45–1,46 и альфа-нафтил эстеразы в 1,41–1,46 раза у самок и самцов природной популяции Nik по сравнению с показателями особей лабораторной культуры Lab UF. Методом секвенирования подтверждено наличие kdr-мутации (L1014F) в гомо- и гетерозиготном состоянии у особей природной популяции, мутация kdr-his (L1014H) не была обнаружена. Полученные результаты позволяют предположить, что у исследованной нами природной популяции M. domestica резистентность к дельтаметрину и высокая чувствительность к хлорфенапиру обусловлены наличием L1014F мутации и повышенной активностью Р450 монооксигеназ. Негативная кросс-резистентность может быть использована для разработки инсектицидных препаратов, снижающих риск быстрого формирования инсектицидной устойчивости M. domestica L.

The purpose of the research is to evaluate the susceptibility to deltamethrin and the pro-insecticide chlorfenapyr in a field population of Musca domestica L. compared to a laboratory strain Lab UF and to clarify a possible mechanism of crossresistance to chlorfenapyr.Materials and methods. The study was carried out on the adults of the laboratory strain Lab UF and the field population Nik of the housefly M. domestica collected from a livestock farm in the Tyumen region, where pyrethroid insecticides had been used for a long time. The toxicity of the pyrethroid deltamethrin (Delcid, 4%) and the pyrrole chlorfenapyr (Pyrafen EC, 360 g/l) against insects was estimated by the no-choice feeding test. Based on the dose-mortality response, lethal concentrations of insecticides were calculated by the probit analysis and the resistance ratio was determined. To clarify the possible mechanism of cross-resistance to chlorfenapyr in the Nik population of M. domestica, the activity of the main detoxification enzymes was determined depending on sex of the insects. In addition, the presence of the kdr-mutation providing resistance to pyrethroids was assessed by the Sanger sequencing.Results and discussion. The lethal concentrations of insecticides and the resistance ratios revealed the moderate resistance to deltamethrin and high susceptibility to chlorfenapyr in the field Nik population. A statistically significant increase in the activity of monooxygenases by 2.25–4.36 times, glutathione-S-transferase by 2.02–2.18 times, acetylcholinesterase by 1.45–1.46 times and alpha-naphthyl esterase by 1.41–1.46 times was noted in females and males of the Nik population compared to these parameters of the Lab UF strain. The presence of the kdr-mutation (L1014F) in houseflies of the field population was confirmed by the Sanger sequencing, while the kdr-his mutation (L1014H) was not detected. The results obtained allow us to suggest that resistance to deltamethrin and high susceptibility to chlorfenapyr in the field population of M. domestica are caused by the L1014F mutation and the increased P450 monooxygenase activity. Negative crossresistance can be used to develop insecticidal formulations that reduce the risk of rapid development of insecticidal resistance in M. domestica L.

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The authors declare that there are no conflicts of interest present.