vniigisРоссийский паразитологический журналRussian Journal of Parasitology1998-84352541-7843ВНИИП – филиал ФГБНУ ФНЦ ВИЭВ РАН10.31016/1998-8435-2023-17-1-91-98vniigis-1011Research ArticleБИОХИМИЯ, БИОТЕХНОЛОГИЯ И ДИАГНОСТИКАBIOCHEMISTRY, BIOTECHNOLOGY AND DIAGNOSTICSСравнительная диагностическая эффективность микроскопии, комбинированной флотации и полимеразной цепной реакции для выявления Giardia spp. у собак и кошекComparative diagnostic efficacy of microscopy, combined flotation and polymerase chain reaction to detect Giardia spp. in dogs and catshttps://orcid.org/0000-0002-3248-8931КурносоваО. П.KurnosovaO. P.

Курносова Ольга Петровна, кандидат биологических наук

117218, Москва, ул. Б. Черемушкинская, 28

 Kurnosova Olga P., Candidate of Biological Sciences 

28, Bolshaya Cheremushkinskaya st., Moscow, 117218, Russia

kurnosova@vniigis.ruЗайцевВ. С.ZaitsevV. S.

Зайцев Валерий Сергеевич, кандидат биологических наук 

107023, Москва, ул. Малая Семеноская, 9/8

 Zaitsev Valery S., Candidate of Biological Sciences 

9/8 Malaya Semenovskaya, Moscow, 107023

vetlabplus@gmail.comhttps://orcid.org/0000-0002-2103-8468АрисовМ. В.ArisovM. V.

Арисов Михаил Владимирович, доктор ветеринарных наук, профессор РАН 

117218, Москва, ул. Б. Черемушкинская, 28

 Arisov Mikhail V., Doctor of Veterinary Sciences, Professor of the RAS 

28, Bolshaya Cheremushkinskaya st., Moscow, 117218, Russia

director@vniigis.ruВсероссийский научно-исследовательский институт фундаментальной и прикладной паразитологии животных и растений – филиал Федерального государственного бюджетного научного учреждения «Федеральный научный центр – Всероссийский научно-исследовательский институт экспериментальной ветеринарии имени К. И. Скрябина и Я. Р. Коваленко Российской академии наук»РоссияAll-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plant – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV” (VNIIP – FSC VIEV)Russian FederationВетеринарная лаборатория ООО «Зайцев плюс»РоссияZaitsev Plus LLC Veterinary LaboratoryRussian Federation2023310320231719198Copyright © Курносова О.П., Зайцев В.С., Арисов М.В., 20232023Курносова О.П., Зайцев В.С., Арисов М.В.Kurnosova O.P., Zaitsev V.S., Arisov M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vniigis.elpub.ru/jour/article/view/1011

Цель исследований – провести сравнительную диагностическую эффективность микроскопии, комбинированного флотационного метода и полимеразной цепной реакции (ПЦР), используемых для выявления Giardia spp. у собак и кошек.Материалы и методы. Сравнение эффективности трех методов выявления Giardia spp. провели на 60 пробах фекалий от собак и кошек из ветеринарной лаборатории «Зайцев +» и «Пастер».Результаты и обсуждение. Наибольшая диагностическая эффективность установлена у ПЦР – 78,3%; диагностическая эффективность комбинированной флотации составила 68,3%; наименьшая диагностическая эффективность установлена у метода микроскопии – 43,3%. Комбинированный флотационный метод выявления гиардий широко используется в лабораторной практике, так как позволяет помимо гиардий выявлять других кишечных паразитов. Микроскопия – наиболее быстрый и простой метод для выявления не только гиардий, но и других кишечных паразитов.

The purpose of the research is to perform a comparative diagnostic efficacy of microscopy, combined flotation method and polymerase chain reaction (PCR) used to detect Giardia spp. in dogs and cats.Materials and methods. The efficacy was compared between three methods to detect Giardia spp. on 60 fecal samples from dogs and cats from the Zaitsev+ and Paster Veterinary Laboratory.Results and discussion. The highest diagnostic efficacy was established for PCR, 78.3%; the diagnostic efficacy of combined flotation was 68.3%; the lowest diagnostic efficacy was found for the microscopy method, 43.3%. The combined flotation method to detect Giardia spp. is widely used in laboratory practice as it allows detection of other intestinal parasites in addition to Giardia spp. Microscopy is the fastest and simplest method for detecting not only Giardia spp. but also other intestinal parasites.

полимеразная цепная реакция (ПЦР)микроскопиякомбинированный флотационный методдиагностическая эффективностьгиардииGiardia spp.собакикошкиpolymerase chain reaction (PCR)microscopycombined flotation methoddiagnostic efficacyGiardiaGiardia spp.dogscatsРабота выполнена в рамках Программы фундаментальных научных исследований в Российской Федерации на долгосрочный период (2021–2030 гг.), составляющей основу государственного задания № FGUG- 2022-0012.The work was carried out within the framework of the Program of Fundamental Scientific Research in the Russian Federation for a long-term period (2021–2030), which forms the basis of state task No. FGUG-2022-0012.ReferencesКурносова О. П. Паразитарные заболевания домашних собак и кошек в мегаполисе Москва // Медицинская паразитология и паразитарные болезни. 2009. № 4. С. 31-35.Kurnosova O. P. Parasitic diseases of domesticated dogs and cats in Moscow. 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The authors declare that there are no conflicts of interest present.